Detection of antigen by immunohistochemical staining

Abstract

The object of the present invention is to provide a method for detecting antigens whereby it is possible to greatly reduce the time required for the detection of antigens, and improve the selection of proper dyes for the detection of given antigens, and which is suitable for the automated detection of antigens. A method for detecting two or more antigens based on multiplicated immunohistochemical staining using colored products or fluorescent dyes for visualizing the multiple antigens is characterized that an inhibitory step against non-specific reactions is performed for coating, with a non-specific reaction inhibiting substance, non-specific reactive substances which would otherwise react with primary antibodies in a non-specific manner, thereby inhibiting the non-specific reaction of those non-specific reactive substances with primary antibodies.

Dependencies are inferred from the claim text.