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US 2005/0032049 A1Published application
Electrochemical method for detecting nucleic acids
OwnerFatima Azek, Pierre Brossier +2inventors
InventorsFatima Azek, Pierre Brossier, Martine Joannes, Benoit Limoges
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Abstract
The invention concerns a novel method for detecting and/or quantifying nucleic acid molecules in a biological sample by electrochemistry and a kit of reagents for implementing said method.
Claims13
A method for detecting and/or assaying nucleic acids in a sample, comprising the following steps:
- a. attaching a nucleic acid to electrodes,
- b. specifically hybridizing a nucleic acid complementary to the attached nucleic acid, said complementary nucleic acid containing a recognition agent,
- c. adding an agent complementary to the recognition agent of (b), said complementary agent being coupled to an enzyme,
- d. adding a substrate for said enzyme such that the action of said enzyme on said substrate leads to the formation of an electroactive compound which can be detected by measuring the variation in faradic current after application of a potential to the electrode.
1 more element
A method for detecting several different organisms in a sample, characterized in that it comprises the following steps:
- a. optional amplification and attachment of the target nucleic acids to the electrodes,
- b. hybridization with the nucleic acids complementary to the targets, each one of them being linked to a different recognition agent,
- c. addition of the agents complementary to the recognition agents, coupled to different labels,
- d. addition of the various substrates for the enzymes in order to generate the various electroactive compounds,
- e. measurement of the faradic current corresponding to the various electroactive compounds, at the surface of the electrode, by applying the potential specific for each compound.
2 more elements
Dependencies are inferred from the claim text.