Multi-chambered pump-valve device

A method for substantially simultaneously treating a plurality of liquid samples, the method comprising:

• a) providing an apparatus that comprises:
• i) a plurality of units, each unit comprising:
• a first chamber having a variable volume,
• a second chamber having a variable volume,
• a third chamber having a variable volume,
• an interconnect comprising one or more passageways, each passageway engaged with one or more chambers so that the interconnect provides fluid communication between the chambers, and
• a treating substance included in at least one of the first chamber, the second chamber, the third chamber or a portion of the interconnect,
• ii) an actuator connected to two or more first chambers for regulating the plurality of first chamber volumes,
• iii) an actuator connected to two or more second chambers for regulating the plurality of second chamber volumes,
• iv) an actuator connected to two or more third chambers for regulating the plurality of third chamber volumes, and
• b) providing a plurality of liquid samples, at least one liquid sample in each of the plurality of first chambers;
• and
• c) controlling the actuator for regulating the plurality of first chamber volumes and controlling the actuator for regulating at least one other plurality of chamber volumes, thereby decreasing the volumes of the first chambers substantially simultaneously and increasing the volumes of at least one other plurality of chambers substantially simultaneously, thereby, in each unit so controlled:
• i) contacting at least a portion of the sample with at least a portion of the treating substance so that the sample is at least partially treated by the treating substance, and
• ii) directing at least a portion of the sample toward the at least one other chamber,
• wherein decreasing the volumes of the first chambers substantially simultaneously and increasing the volumes of at least one other plurality of chambers substantially simultaneously causes substantially simultaneous treatment of the plurality of samples.