Reading multi-featured arrays
AssigneeAgilent Technologies, Inc.
InventorsAndreas Dorsel, Glenda Delenstarr, Kenneth Staton, George Tsai
Life of this patent
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Abstract
A method, apparatus, and computer program product for reading fluorescence signals from an array of chemical moieties (such as different sequence peptides or polynucleotides, for example different DNA sequences). In the method the spatial sequence of scanned locations need not be the same as the temporal sequence. For example, a later illuminated line may be spatially closer to an earlier illuminated line than is a temporally intervening illuminated line.
Claims19
An apparatus for reading fluorescence signals from an array of chemical moieties, comprising:
- (a) an illumination source to cause fluorescence of the chemical moieties;
- (b) a scan system to direct the illumination source to different locations on the array;
- and
- (c) a detector to detect any resulting fluorescence from the array;
- (c) a processor which controls the scan system such that multiple locations on the array are illuminated and any resulting fluorescence detected, wherein a later illuminated location is spatially closer to an earlier illuminated location than is a temporally intervening illuminated location lying on a same line as the later and earlier illuminated locations.
An apparatus for reading fluorescence signals from an array of chemical moieties, comprising:
- (a) an illumination source to cause fluorescence of the chemical moieties;
- (b) a scan system to direct the illumination source to different locations on the array;
- and
- (c) a detector to detect any resulting fluorescence;
- (c) a processor which controls the scan system such that multiple paths across the array are illuminated and any resulting fluorescence detected, wherein the paths extend in a same lengthwise direction and are spaced from one another in a crosswise direction, and the spatial sequence of the paths does not correspond to their temporal sequence.
An apparatus for reading fluorescence signals from an array of chemical moieties, comprising:
- (a) an illumination source to cause fluorescence of the chemical moieties;
- (b) a scan system to direct the illumination source to different locations on the array;
- and
- (c) a detector to detect any resulting fluorescence from the array;
- (c) a processor which controls the scan system such that multiple parallel lines across the array are illuminated and any resulting fluorescence detected, wherein a later illuminated line is closer to an earlier illuminated line than a temporally intervening illuminated line.
A computer program product, comprising:
- a computer readable storage medium having a computer program stored thereon which, when loaded into a computer communicating with an apparatus for reading fluorescence signals from an array of chemical moieties, performs the steps of:
- illuminating multiple locations on the array and detecting any resulting fluorescence, wherein a later illuminated location is spatially closer to an earlier illuminated location than is a temporally intervening illuminated location lying on a same line as the later and earlier illuminated locations.
A computer program product, comprising:
- a computer readable storage medium having a computer program stored thereon which, when loaded into a computer communicating with an apparatus for reading fluorescence signals from an array of chemical moieties, performs the steps of:
- illuminating multiple parallel lines across the array and detecting any resulting fluorescence from the array, wherein a later illuminated line is closer to an earlier illuminated line than a temporally intervening illuminated line.
Dependencies are inferred from the claim text.