A Novel Method for Production of Transformed Dihaploid Corn Plants
AssigneeMonsanto Technology LLC
InventorsCharles L. Armstrong, Carl Frederick Behr, Gurdip S. Brar, David R. Duncan, Terry Foley, Lorelei C. Marshall
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Abstract
The present invention relates to a novel system for generating transformed dihaploid corn plants. In particular, the invention relates to the production of haploid corn callus, transformation of that tissue, dihaploidization, and regeneration of transgenic plants. Transgenic dihaploid corn plants can then be easily produced.
Claims27
A method of obtaining a transformed dihaploid plant comprising:
- obtaining haploid sporophytic tissue;
- transforming the haploid sporophytic tissue;
- producing dihaploid tissue from the transformed haploid sporophytic tissue;
- and regenerating a dihaploid plant from the dihaploid tissue.
A method of obtaining a transformed dihaploid plant comprising:
- obtaining haploid sporophytic tissue;
- transforming the haploid sporophytic tissue;
- regenerating a haploid plant;
- and
- producing a dihaploid plant from the haploid plant.
A method of obtaining a transformed dihaploid plant comprising:
- obtaining haploid tissue;
- culturing the haploid tissue to form haploid callus;
- transforming the haploid callus;
- producing dihaploid callus from the transformed haploid callus;
- and
- regenerating a dihaploid plant from the dihaploid callus.
A method of obtaining a transformed dihaploid corn plant comprising:
- obtaining haploid corn tissue;
- culturing the haploid corn tissue to form haploid callus;
- transforming the haploid callus;
- producing dihaploid corn callus from the transformed haploid corn callus;
- and regenerating a dihaploid corn plant from the dihaploid callus.
A method of obtaining a transformed dihaploid corn plant comprising:
- obtaining haploid corn tissue;
- culturing the haploid corn tissue to form haploid callus;
- transforming the haploid callus;
- regenerating a haploid plant from the transformed haploid corn callus;
- and producing a dihaploid corn plant from the haploid corn plant.
A method of obtaining a transformed dihaploid corn plant comprising:
- obtaining haploid corn tissue;
- culturing the haploid corn tissue to form haploid multiple bud cultures;
- transforming the multiple bud cultures;
- producing dihaploid multiple bud cultures from the transformed multiple bud cultures;
- and
- regenerating a dihaploid corn plant from the dihaploid multiple bud cultures.
Dependencies are inferred from the claim text.