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AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsMario H. Skiadopoulos, Brian R. Murphy, Peter L. Collins
Estimating the term…
Recombinant human parainfluenza virus type 2 (HPIV2) viruses and related immunogenic compositions and methods are provided. The recombinant HPIV2 viruses, including HPIV2 chimeric and chimeric vector viruses, provided according to the invention are infectious and attenuated in permissive mammalian subjects, including humans, and are useful in immunogenic compositions for eliciting an immune responses against one or more PIVs, against one or more non-PIV pathogens, or against a PIV and a non-PIV pathogen. Also provided are isolated polynucleotide molecules and vectors incorporating a recombinant HPIV2 genome or antigenome.
A method for producing an infectious, self-replicating, recombinant human parainfluenza virus type 2 (HPIV2) from one or more isolated polynucleotide molecules encoding said HPIV2, comprising:
An isolated, infectious, self-replicating, recombinant human parainfluenza virus type 2 (HPIV2) comprising a PIV major nucleocapsid (N) protein, a PIV nucleocapsid phosphoprotein (P), a PIV large polymerase protein (L), and a partial or complete, polyhexameric recombinant HPIV2 genome or antigenome.
An immunogenic composition comprising an immunogenically effective amount of an isolated, infectious, self-replicating, recombinant human parainfluenza virus type 2 (HPIV2) in a pharmaceutically acceptable carrier, said HPIV2 comprising a polyhexameric, recombinant HPIV2 genome or antigenome, a PIV N protein, a PIV P protein, and a PIV L protein.
130-182. Cancelled.
A method for stimulating the immune system of a mammalian subject to induce an immune response in the subject against PIV which comprises administering to the subject an immunologically sufficient amount of an isolated, infectious, self-replicating, recombinant human parainfluenza virus type 2 (HPIV2) comprising a PIV major nucleocapsid (N) protein, a PIV nucleocapsid phosphoprotein (P), a PIV large polymerase protein (L), and a partial or complete, polyhexameric recombinant HPIV2 genome or antigenome.
184-231. Cancelled
A method for sequential immunization to stimulate the immune system of a mammalian subject to induce an immune response against multiple pathogens comprising administering to the subject an immunologically sufficient amount of a first HPIV and subsequently administering to the subject an immunologically sufficient amount of a second HPIV, wherein at least one of said first and second HPIVs comprises a partial or complete HPIV2 vector genome or antigenome combined with one or more heterologous gene(s) or genome segment(s) encoding one or more antigenic determinant(s) of one or more heterologous pathogen(s) to form a chimeric HPIV1 genome or antigenome.
233-254. Cancelled.
An isolated polynucleotide comprising a partial or complete, polyhexameric recombinant human parainfluenza virus type 2 (HPIV2) genome or antigenome modified by one or more attenuating mutations that are recombinantly introduced into said HPIV2 genome or antigenome.
256-277. Cancelled.
An expression vector comprising an operably linked transcriptional promoter, a polynucleotide sequence comprising a partial or complete, polyhexameric recombinant human parainfluenza virus type 2 (HPIV2) genome or antigenome modified by one or more attenuating mutations that are recombinantly introduced into said HPIV2 genome or antigenome, and a transcriptional terminator.
279-294. Cancelled.
Dependencies are inferred from the claim text.