Process for generating multilayered particles
AssigneeMirus Bio Corporation
InventorsVladimir Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein
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Abstract
We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
Claims20
1-5. (canceled)
A process for delivering a polynucleotide to a cell comprising:
- condensing a first polyanion with a polycation to form a particle and adding a second polyanion to the particle to form a complex wherein at least one of the polyanions is the polynucleotide;
- and, contacting the cell with the complex.
A process for delivering a polynucleotide to a cell comprising:
- a) adding a solution containing a polynucleotide to a solution containing an excess of polycation wherein the polynucleotide and the polycation interact to form a particle;
- b) adding a solution containing an excess of polyanion to the particle wherein the particle and the polyanion interact to form a complex;
- and,
- c) contacting the cell with the complex.
A complex for delivering a polynucleotide to a cell, comprising:
- three or more layers of polyanions of alternating charge wherein at least one of the polyions is the polynucleotide.
Dependencies are inferred from the claim text.