US 2004/0203101 A1Published application

Brain-associated inhibitor of tissue-type plasminogen activator

AssigneesHuman Genome Sciences, Inc., American Red Cross

InventorsGregg A. Hastings, Timothy A. Coleman, Patrick J. Dillon, Daniel A. Lawrence, Maria Sandkvist, Manuel Yepes, Michael K. K. Wong

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Abstract

The present invention relates to a novel BAIT protein which is a member of serpin superfamily which is expressed primarily in brain tissue. In particular, isolated nucleic acid molecules are provided encoding the human and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of BAIT activity. Also provided are diagnostic methods for detecting nervous system-related disorders and therapeutic methods for treating nervous system-related disorders. Additionally, the present invention is related to methods of treating patients with BAIT polynucleotides or polypeptides, wherein said patients have had seizures or epilepsy.

Claims36

Dependent claims

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 99% identical to a sequence selected from the group consisting of:

(a) a nucleotide sequence encoding the BAIT (Brain-Associated Inhibitor of Tissue-Type Plasminogen Activator) polypeptide having the complete amino acid sequence in FIG. 1 (SEQ ID NO:2);
(b) a nucleotide sequence encoding the mature BAIT polypeptide having the amino acid sequence at positions 19 to 410 in FIG. 1 (SEQ ID NO:2);
(c) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n-410 of SEQ ID NO:2, where n is an integer in the range of 2-49;
(d) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues 1-m of SEQ ID NO:2, where m is an integer in the range of 381-409;
(e) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n-m of SEQ ID NO:2, where n is an integer in the range of 2-49 and m is an integer in the range of 381-409;
(f) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete BAIT amino acid sequence encoded by the cDNA clone contained in ATCC Deposit 97722 wherein said portion excludes up to 48 amino acids from the amino terminus and up to 30 amino acids from the C-terminus of said complete amino acid sequence;
(g) a complete nucleotide sequence as shown in FIG. 1 (SEQ ID NO:1);
(h) a complete nucleotide sequence as shown in FIG. 1 (SEQ ID NO:1) wherein said nucleotide sequence encodes the BAIT polypeptide having the complete amino acid sequence in FIG. 1 (SEQ ID NO:2);
(i) a nucleotide sequence as shown in FIG. 1 (SEQ ID NO:1) wherein said nucleotide sequence encodes the mature BAIT polypeptide having the amino acid sequence in FIG. 1 (SEQ ID NO:2);
(j) a nucleotide sequence of the cDNA clone contained in ATCC Deposit No. 97722;
(k) a nucleotide sequence encoding the BAIT polypeptide having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97722;
(l) a nucleotide sequence encoding the mature BAIT polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97722;
(m) a nucleotide sequence comprising a polynucleotide which encodes the amino acid sequence of an epitope-bearing portion of a BAIT polypeptide having an amino acid sequence in (a), (b), (c), (d), (f), (g), (i), or (j);
and,
(n) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d), (e), (f), (h), (i), (k), (l), or (m).

12 more elements

An isolated BAIT polypeptide having an amino acid sequence at least 97% identical to a sequence selected from the group consisting of:

(a) the amino acid sequence of the BAIT polypeptide having the complete amino acid sequence in SEQ ID NO:2;
(b) the amino acid sequence of the mature BAIT polypeptide having the amino acid sequence at positions 19 to 410 in SEQ ID NO:2;
(c) amino acid residues n to 410 of SEQ ID NO:2, where n is an integer in the range of 2 to 49;
(d) amino acid residues 1 to m of SEQ ID NO:2, where m is an integer in the range of 381 to 410;
(e) amino acid residues n to m of SEQ ID NO:2, where n is an integer in the range of 2 to 49 and m is an integer in the range of 381 to 410;
(f) a polypeptide comprising amino acids 342-378 of SEQ ID NO:2;
(g) the amino acid sequence of the BAIT polypeptide having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97722;
(h) the amino acid sequence of the complete polypeptide, excluding the N-terminal methionine residue, which amino acid sequence is encoded by the cDNA clone contained in ATCC Deposit No. 97722;
(i) the amino acid sequence of the mature BAIT polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97722;
(j) a portion of the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit 97722 wherein said portion excludes up to 48 amino acid residues from the amino terminus of said complete amino acid sequence;
(k) a portion of the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit 97722 wherein said portion excludes up to 30 amino acid residues from the C-terminus of said complete amino acid sequence;
(l) a portion of the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit 97722 wherein said portion excludes up to 48 amino acid residues from the amino terminus and up to 30 amino acids from the C-terminus of said complete amino acid sequence;
and,
(m) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), or (l).

11 more elements

Dependencies are inferred from the claim text.

Facts

Application: 10/752,041Filed 7 Jan 2004
Priority: 11 Oct 1996
Published: 14 Oct 2004
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

C07K 14/8132
Serpins: plasminogen activator inhibitors
C07K 14/8121
Serine protease inhibitors: serpins
A61K 38/00
Medicinal preparations containing peptides
C07K
Peptides

Family

Application60/028,117Us provisional application · filed 11 Oct 1996
Application08/948,997Division · filed 10 Oct 1997
Application60/123,704Us provisional application · filed 10 Mar 1999
Application09/348,817Division · filed 8 Jul 1999
Application09/521,664Continuation · filed 8 Mar 2000
Application60/247,971Us provisional application · filed 14 Nov 2000
Application09/722,292Continuation in part · filed 28 Nov 2000
Application09/722,292Division · filed 28 Nov 2000
Application09/957,485Continuation in part · filed 21 Sep 2001
Application09/957,485Continuation · filed 21 Sep 2001
Application09/957,485Division · filed 21 Sep 2001
Application09/987,021Continuation in part · filed 13 Nov 2001
Application10/355,208Continuation in part · filed 31 Jan 2003
Application10/355,208Division · filed 31 Jan 2003
Application10/752,041Continuation in part · filed 7 Jan 2004
US 2002/0143165 A1Published application
US 2002/0165147 A1Published application
US 2004/0038880 A1Published application
US 6,008,020 ARelated patent
US 6,191,260 B1Related patent
US 6,541,452 B1Related patent
US 7,087,574 B2Related patent
US 7,235,529 B2Related patent
US 2004/0203101 A1This patent

From the US filing history. Foreign family members aren’t listed.

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