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AssigneeRiken
InventorsKaori Tajima, Takanori Kigawa, Mikako Shirouzu, Takashi Yabuki, Goushi Ishihara, Shigeyuki Yokoyama
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A method for producing a protein using a cell-free protein synthesis system comprising a detergent so that the protein can be synthesized without aggregation, is provided. The protein is a protein comprising a hydrophobic region in at least a portion thereof, for example, a membrane protein or its fragment (portion). And the detergent is a mild detergent which would not denature the protein, for example, a nonionic or amphoteric ionic detergent.
A method for producing a protein using a cell-free protein synthesis system comprising a detergent to synthesize the protein without aggregation.
A method for producing a protein in a cell-free protein synthesis system using a bacterial cell extract, wherein said system comprises a detergent to synthesize a membrane protein without aggregation.
A method for reconstituting a protein produced in a cell-free protein synthesis system comprising a nucleic acid template coding for at least a portion of a membrane protein, a detergent, and a lipid,
A method for producing a membrane protein comprising the steps of:
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