Novel human phosphodiesterase IV isozymes
AssigneePfizer Inc.
InventorsDouglas A. Fisher, Michael D. Robbins
Life of this patent
Estimating the term…
Abstract
This invention relates to novel nucleic acid sequences encoding three novel human phosphodiesterase (hPDE IV) isozymes. It also relates to polypeptides encoded by such sequences. This invention also relates to an assay method for detecting the presence of such novel isozymes in human cells, and to a method of identifying compounds or other substances that inhibit or modify the activity of such isozymes.
Claims18
An DNA segment comprising the DNA sequence of:
- (a) SEQUENCE ID NO. 1 or an alleleic variation thereof;
- (b) SEQUENCE ID NO. 2 or an alleleic variation thereof;
- or
- (c) SEQUENCE ID NO. 3 or an alleleic variation thereof.
A substantially purified polypeptide comprising the amino acid sequence of SEQUENCE ID NO. 4, SEQUENCE ID NO. 5 or SEQUENCE ID NO. 6.
A polypeptide produced by expression in a host cell into which has be n incorporated the DNA sequence of:
- (a) SEQUENCE ID NO. 1 or an alleleic variation thereof;
- (b) SEQUENCE ID NO. 2 or an alleleic variation thereof;
- or
- (c) SEQUENCE ID NO. 3 or an alleleic variation thereof.
An isolated DNA segment comprising the genomic promoter region that regulates transcription or translation of:
- (a) the DNA sequence of SEQUENCE ID NO. 1 or an alleleic variation thereof;
- or
- (b) th DNA sequence of SEQUENCE ID NO. 2 or an alleleic variation thereof;
- or
- (c) the DNA sequence of SEQUENCE ID NO. 3 or an alleleic variation thereof.
A method of detecting the presence of hPDE IV-B1, hPDE IV-B2 or hPDE IV-B3 in human cells comprising:
- (a) performing a reverse transcriptase-polymerase chain reaction on total RNA from such cells using a pair of polymerase chain reaction primers that are specific for, respectively, hPDE IV-B1, hPDE IV-B2 or hPDE IV-B3, as determined from, respectively:
- (i) the DNA sequence of SEQUENCE ID NO. 1 or an alleleic variation thereof;
- or
- (ii) the DNA sequence of SEQUENCE ID NO. 2 or an alleleic variation thereof;
- or
- (iii) the DNA sequence of SEQUENCE ID NO. 3 or an alleleic variation thereof;
- and
- (b) assaying the appearance of an appropriately sized PCR fragment by agarose gel electrophoresis.
An isolated DNA segment comprising a DNA sequence that is a subset of the DNA sequence of SEQUENCE ID NO. 1, or an alleleic variation thereof, and that is capable of hybridizing to the DNA sequence of SEQUENCE ID NO. 1, or an alleleic variation thereof, when used as a probe, or amplifying all or part of such sequence when used as a polymerase chain reaction primer.
An isolated DNA segment comprising a DNA sequence that is a subset of the DNA sequence of SEQUENCE ID NO. 2, or an alleleic variation thereof, and that is capable of hybridizing to the DNA sequence of SEQUENCE ID NO. 2, or an alleleic variation thereof, when used as a probe, or amplifying all or part of such sequence when used as a polymerase chain reaction primer.
An isolated DNA segment comprising a DNA sequence that is a subset of the DNA sequence of SEQUENCE ID NO. 3, or an alleleic variation thereof, and that is capable of hybridizing to the DNA sequence of SEQUENCE ID NO. 3, or an alleleic variation thereof, when used as a probe, or amplifying all or part of such sequence when used as a polymerase chain reaction primer.
Dependencies are inferred from the claim text.