Surface modification, linker attachment, and polymerization methods
OwnerZbigniev Skrzypcznski, Raymond F. Cracauerinventors
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Abstract
The present invention relates to surface modifications and linker attachments. For example, the present invention provides surface modification and linker chemistry that facilitates manufacture and use of microarrays, including nucleic acid and protein microarrays. The present invention also relates to array spotting through non-aqueous liquids.
Claims23
A composition comprising a surface, said surface comprising a coating, said coating comprising a linker, wherein said linker has a first end covalently coupled to said surface and a second end comprising a reactive group, wherein said linker further comprises a hydrophobic portion and a hydrophilic portion, wherein said hydrophobic portion is configured to collapse in an aqueous environment so as to increase stability of attachment of said linker to said surface.
A composition comprising a surface, said surface comprising a hydrophobic coating, said hydrophobic coating comprising a plurality of oxidize spots, said oxidized spots produced by a method comprising:
- a) coating said surface with compounds containing disulfide bonds to generate said hydrophobic coating;
- and
- b) exposing said hydrophobic coating in a plurality of spots with an oxidizing agent to generate said plurality of oxidized spots.
A method comprising;
- a) providing;
- i) a solid support comprising a well,
- ii) a non-aqueous liquid, and
- iii) a detection reagent solution;
- and
- b) adding said non-aqueous liquid to said well, and
- c) adding said detection reagent solution to said well through said non-aqueous liquid under conditions such that at least one microarray-spot is formed in said well.
Dependencies are inferred from the claim text.