Directed evolution of enzymes and antibodies
AssigneeBoard of Regents, The University of Texas System
InventorsBrent Iverson, George Georgiou, Gang Chen, Mark J. Olsen, Patrick S. Daugherty
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Abstract
The invention relates to methods of selecting proteins, out of large libraries, having desirable characteristics. Exemplified are methods of expressing enzymes and antibodies on the surface of host cells and selecting for desired activities. These methods have the advantage of speed and ease of operation when compared with current methods. They also provide, without additional cloning, a source of significant quantities of the protein of interest.
Claims38
A method for selecting a polypeptide from a plurality of candidate proteins, the method comprising the steps of:
- (a) obtaining a library of vectors that encode a plurality of distinct candidate polypeptides, wherein said vector provides for the cell surface expression of said candidate polypeptides;
- (b) expressing each of said plurality of candidate polypeptides on the surface of a host cell;
- and
- (c) selecting a host cell that expresses a desired polypeptide.
A method for catalyzing a chemical reaction, comprising the steps of:
- (d) obtaining a host cell that expresses an enzyme or catalytic antibody on the surface of the outer membrane;
- and
- (e) contacting said host cell with a sample containing the necessary substrates for said chemical reaction.
Dependencies are inferred from the claim text.