Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
AssigneeZimmer Ortho Biologics, Inc.
InventorKevin Thorne
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Abstract
A method for purifying bone-derived osteogenic proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recovery process. The high and low ultrafiltration processes preferably select proteins having a nominal molecular weight between approximately 8 kilodaltons and approximately 50 kilodaltons. Processes of the present invention may be used to recover osteogenic proteins from bone demineralization waste streams.
Claims24
A process for obtaining osteogenic proteins from mammalian bone tissue comprising:
- contacting bone tissue with an acidic demineralization medium to provide demineralized bone tissue and a mineralized supernatant solution;
- separating the mineralized supernatant solution from the demineralized bone tissue;
- removing at least part of the mineral component of the mineralized supernatant solution to provide a protein supernatant solution;
- extracting osteogenic proteins from the protein supernatant solution by contacting the protein supernatant solution with a protein extraction agent to provide an extracted protein medium;
- and
- recovering osteogenic proteins from the extracted protein medium.
A method for isolating osteogenic proteins from mammalian bone tissue comprising:
- demineralizing bone tissue in an acid medium to provide demineralized bone tissue and a mineral-containing acid supernatant;
- removing at least a portion of the minerals from the mineral-containing acid supernatant to provide a protein supernatant solution;
- extracting osteogenic proteins from the protein supernatant solution with a protein extraction agent to provide an extracted protein medium;
- and
- recovering osteogenic proteins from the extracted protein medium.
A method for isolating osteogenic proteins from mammalian bone tissue comprising:
- demineralizing bone tissue in an acid medium to provide demineralized bone tissue and a mineral-containing acid supernatant;
- extracting osteogenic proteins from the protein supernatant solution with a protein extraction agent to provide an extracted protein medium;
- and
- recovering osteogenic proteins from the extracted protein medium.
Dependencies are inferred from the claim text.