US 2004/0072303 A1 · Method of preparing a guanosine-group compound and an intermediate thereof

Abstract

A glyoxal-guanosine-group compound is prepared either by reacting glyoxal-guanine with any one of ribose-1-phosphate and 2-deoxyribose-1-phosphate in the presence of purine nucleoside phosphorylase, or by reacting glyoxal-guanine with any one selected from the group consisting of uridine, 2′-deoxyuridine and thymidine, together with phosphate ion, in the presence of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase. The glyoxal-guanosine-group compound is then decomposed by alkali, whereby a guanosine-group compound consisting of guanosine and 2′-deoxyguanosine is prepared.

Claims10

Dependent claims

A method of preparing a guanosine-group compound, which comprises the steps of:

reacting glyoxal-guanine represented by formula (1):
13
with ribose-1-phosphate or 2-deoxyribose-1-phosphate in the presence of purine nucleoside phosphorylase, thereby obtaining a compound represented by formula (2):
14
wherein R represents a hydrogen atom or a hydroxyl group;
and
decomposing, by alkali, the compound represented by formula (2), thereby obtaining guanosine or 2′-deoxyguanosine.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/615,265Filed 8 Jul 2003

Priority

JP 2000-08730227 Mar 2000

Published

15 Apr 2004

Term ends

Not estimatedThe source records don’t support an estimate.

Classification2 codes

C12P 19/40
Nucleosides: having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
C12P
Fermentation or enzyme-using processes to synthesise a desired chemical compound or composition

Method of preparing a guanosine-group compound and an intermediate thereof

Life of this patent

Method of preparing a guanosine-group compound and an intermediate thereof

Life of this patent

Abstract

Claims10