Class II cytokine receptor-7
AssigneeZymoGenetics, Inc.
InventorsSi Lok, Choon J. Kho, Anna C. Jelmberg, Robyn L. Adams, Theodore E. Whitmore, Theresa M. Farrah
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Abstract
Novel receptor polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed. The polypeptides comprise an extracellular domain of a cell-surface receptor that is expressed in kidneys, pancreas, prostate, adrenal cortex and nervous tissue. The polypeptides may be used within methods for detecting ligands that promote the proliferation and/or differentiation of these organs.
Claims21
An isolated polynucleotide encoding a ligand-binding receptor polypeptide, said polypeptide comprising a sequence of amino acids selected from the group consisting of:
- (a) residues 30 to 250 of SEQ ID NO:2;
- (b) allelic variants of (a);
- and
- (c) sequences that are at least 80% identical to (a) or (b).
An isolated polynucleotide encoding a polypeptide selected from a group defined SEQ ID NO:2 consisting of residues 1 to 250, residues 1 to 274, residues 1 to 553, residues 2 to 250, residues 2 to 274, residues 2 to 553, residues 251 to 274, residues 251 to 553 and residues 275 to 553.
An expression vector comprising the following operably linked elements:
- a transcription promoter;
- a DNA segment encoding a ligand-binding receptor polypeptide, said polypeptide comprising a sequence of amino acids selected from the group consisting of:
- (a) residues 30 to 250 of SEQ ID NO:2;
- (b) allelic variants of (a);
- and
- (c) sequences that are at least 80% identical to (a) or (b);
- and
- a transcription terminator.
An expression vector comprising the following operably linked elements:
- (a) a transcription promoter;
- (b) a DNA segment encoding a chimeric polypeptide, wherein said chimeric polypeptide is comprised of a first portion and a second portion joined by a peptide bond, said first portion consisting essentially of a ligand binding domain of a receptor polypeptide selected from the group consisting of:
- (i) a receptor polypeptide as shown in SEQ ID NO:2;
- (ii) allelic variants of SEQ ID NO:2;
- and
- (iii) receptor polypeptides that are at least 80% identical to (i) or (ii), and said second portion consisting essentially of an affinity tag;
- and
- (c) a transcription terminator.
Dependencies are inferred from the claim text.