Cancer-linked genes as targets for chemotherapy
OwnerPaul E Young, Stephen K Horrigan +2inventors
InventorsPaul E Young, Stephen K Horrigan, Zoe Weaver, Gregory Endress
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Abstract
Cancer-linked gene sequences, and derived amino acid sequences, are disclosed along with processes for assaying potential antitumor agents based on their modulation of the expression of these cancer-linked genes. Also disclosed are antibodies that react with the disclosed polypeptides and methods of diagnosing and treating cancer using the gene sequences. A novel gene and polypeptide are also disclosed.
Claims32
A process for identifying an agent that modulates the activity of a cancer-related gene comprising:
- (a) contacting a compound with a cell containing a gene that corresponds to a polynucleotide having a sequence selected from the group consisting of SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 17 and 19 and under conditions promoting the expression of said gene;
- and
- (b) detecting a difference in expression of said gene relative to when said compound is not present
- thereby identifying an agent that modulates the activity of a cancer-related gene.
A process for determining the cancerous status of a cell, comprising determining an increase in the level of expression in said cell of at least one gene that corresponds to a polynucleotide having a sequence selected from the group consisting of SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 17 and 19 wherein an elevated expression relative to a known non-cancerous cell indicates a cancerous state or potentially cancerous state.
An antibody that reacts with a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20.
A process for treating cancer comprising contacting a cancerous cell with an agent having activity against an expression product encoded by a gene sequence selected from the group consisting of SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 17 and 19.
An immunogenic composition comprising a polypeptide comprising an amino acid sequence with at least 90% identity to a sequence selected from the group consisting of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 and wherein and amino acid difference results only from conservative amino acid substitutions.
An isolated polynucleotide comprising a polynucleotide having at least 95% sequence identity to a member selected from the group consisting of SEQ ID NO:
- 3 or the complement thereof.
An isolated polynucleotide comprising a polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding the amino acid sequence of SEQ ID NO:
- 4, and
- (b) the complement of (a).
An isolated polynucleotide comprising an amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO:
- 4 and wherein any difference in sequence identity results only from conservative amino acid substitutions.
Dependencies are inferred from the claim text.