Detection of genetic polymorphisms
AssigneeRiken Institute of Physical and Chemical Research
InventorsYusuke Nakamura, Akihiro Sekine, Aritoshi Ilda, Susumu Saito
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Abstract
The present invention relates to genetic polymorphism data, compositions and methods for detecting genetic polymorphisms, methods for evaluating drugs using genetic polymorphisms and screening methods for drugs.
Claims123
A method of identifying individuals having a polymorphism, comprising;
- a. providing nucleic acid from a subject;
- and
- b. detecting the presence of at least one polymorphism in said nucleic acid, said at least one polymorphism selected from the group consisting of polymorphisms found in SEQ ID Nos:1-3360 and 3361-7669.
A composition comprising a nucleic acid, said nucleic acid comprising a sequence selected from the group consisting of SEQ ID NO:
- 1-3360 and 3361-7669 or complements thereof.
A kit for detecting a polymorphism, comprising at least one reagent that specifically detects a polymorphism in a sequence selected from the group consisting of SEQ ID Nos:3360 and 3361-7669.
A method for screening subjects for genetic markers associated with drug metabolizing enzyme(s), comprising:
- a) providing a biological sample comprising a nucleic acid from a subject;
- b) testing said nucleic acid for a polymorphism in a genetic marker associated with a drug metabolizing enzyme, said genetic marker comprising one or more nucleotide polymorphisms designated by n, said n selected from a base substitution, an insertion, or a deletion found in a sequence selected from the group consisting of SEQ ID Nos:1-3360 and 3361-7669.
A composition comprising an array of detection assays, said array comprising a plurality of drug metabolizing enzyme nucleotide polymorphism detection assays, one or more of said detection assays being capable of detecting one or more nucleotide polymorphisms designated by n in SEQ ID Nos:1-3360 and 3361-7669, wherein n represents a base substitution, insertion, or deletion compared to a wild-type sequence.
A composition comprising a detection probe for determining the presense or absence a single nucleotide polymorphism in a gene encoding a drug metabolizing enzyme, said gene comprising a sequence selected from the group consisting of SEQ ID Nos:1-3360 and 3361-7669.
A method of determining the effectiveness of or side-effect of a drug or treatment protocol, comprising;
- a. administering a drug or treatment protocol to one or more subjects;
- b. obtaining nucleic acid from said one or more subjects;
- c. using a detection assay to detect the presence of at least one polymorphism in said nucleic acid from said one or more of subjects, said at least one polymorphism selected from the group consisting of polymorphisms found in SEQ ID Nos:1-3360 and 3361-7669;
- and,
- d. assigning an effectiveness rating, side-effect rating, or score for said drug or treatment protocol based upon a result of one or more said detection assays.
A method of prescribing a drug to or treatment protocol for a subject, comprising;
- a. providing nucleic acid from said subject;
- b. using a detection assay to detect the presence of at least one polymorphism in said nucleic acid, said at least one polymorphism selected from the group consisting of polymorphisms found in SEQ ID Nos:1-3360 and 3361-7669;
- and,
- c. prescribing said drug or treatment protocol based upon the result of said detection assay.
A method for generating assay data comprising:
- a. obtaining a sample from a subject containing nucleic acid;
- b. transferring said sample to a laboratory;
- and
- c. receiving data from said laboratory, wherein said data corresponds to the presence of at least one polymorphism in said nucleic acid, said at least one polymorphism selected from the group consisting of polymorphisms found in SEQ ID Nos:1-3360 and 3361-7669.
A composition comprising a nucleic acid, said nucleic acid comprising a gene sequence, said gene sequence having at least one polymorphism, said at least one polymorphism selected from the group consisting of polymorphisms found in SEQ ID NOs:1-3360 and 3361-7669.
A composition comprising an oligonucleotide, said oligonucleotide comprising at least one polymorphism, said at least one polymorphism selected from the group consisting of polymorphisms found in SEQ ID Nos:1-3360 and 3361-7669, and said oligonucleotide comprising at least five bases upstream or five bases downstream of said polymorphism.
Dependencies are inferred from the claim text.