Double fructan beets
AssigneeSES Europe N.V./S.A.
InventorsGuy Weyens, Jean Lathouwers, Cornelius (Kees) Van Dun
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Abstract
The present invention is in the field of fructan producing plants. Furthermore the present invention is directed to the production of at least 3 degree of polymermization. Additionally, the present invention is more specifically directed to non fructan producing plant which are transformed or crossed to contain at least two genes of the three fructosyltransferase.
Claims18
A method for producing fructans having a low degree of polymerization, comprising the steps of:
- (a) selecting two different nucleic DNA which code respectively for two fructosyltransferase enzymes which convert at least one substrate into a fructan having a low degree of polymerization;
- (b) linking the DNA to suitable transcription-initiation and transcription-termination signals to provide an expression construct;
- (c) transforming a suitable host plant with the expression construct regenerating a transgenic plant from the transformed plant cell,
- (d) cultivating the transgenic plant under conditions enabling the expression of the two fructosyltransferase enzymes during biosynthesis of the fructan,
- (e) isolating the fructan from the transgenic plant.
A method for producing seeds of transgenic plants which accumulate fructans having a degree of polymerisation at or above DP3, comprising the steps of:
- (a) selecting two different nucleic DNA which code respectively for two fructosyltransferase enzymes mediates the biosynthesis of a fructan having a low degree of polymerization;
- (b) linking the DNA to suitable transcription-initiation and transcription-termination signals to provide an expression construct;
- (c) transforming a suitable plant cell with the expression construct regenerating a transgenic plant from the transformed plant cell,
- (d) cultivating the transgenic plant under conditions enabling the expression of the two fructosyltransferase enzymes
- (e) producing seed of transgenic plants which express the two fructosyltransferase enzymes,
A transgenic plant which accumulates fructans having a degree of polymerisation at or above DP2 comprising:
- two different nucleic DNA operatively linked to a suitable transcription-initiation and transcription-termination signal to provide an expression which code respectively for two fructosyltransferase enzymes which convert at least one substrate into a fructan having at least a DP2.
A transgenic plant which accumulates carbohydrates as fructans which is made from a host plant that when in the wildtype does not accumulate fructans said plant comprising:
- the degree of fructosyltransferase activity determined as the percentage of the degree of polymerisation at DP2 and having a degree of fructosyltransferase activity determined as the percentage of the degree of polymerisation at DP3, and having a degree of fructosyltransferase activity determined as the percentage of the degree of polymerisation at DP4, and having a degree of fructosyltransferase activity determined as the percentage of the degree of polymerisation at DP5, wherein at least two different nucleic acids encoding fructosyltransferase are present in the transgenic plant that are not present in the wildtype host plant.
A transgenic plant comprising:
- at least two genes that encode fructosyltransferase wherein the fructosyltransferase activity has a degree of polyermization above DP 2.
Dependencies are inferred from the claim text.