Method of cloning animals

A method of cloning an animal with a cell at G1-phase of the cell cycle comprising the steps of:

• a)culturing animal cells for a period of time sufficient to allow said cells to reach confluence and G1-phase of the cell cycle;
• b)introducing the whole cell and/or genome of said cultured cells of step a) into enucleated oocyte to obtain reconstructed embryos;
• and
• c)developing said reconstructed embryo of step b) to obtain an animal.

A method of cloning an animal comprising the steps of:

• a) culturing animal cells for a period of time sufficient to allow said cells to reach confluence and G1-phase of cell cycle;
• b) enucleating maturing oocyte between 18 to 26 hours of maturation and allowing said enucleated oocyte to mature for an additional period of time between 2 to 10 hours, or enucleating an oocyte between 26 to 34 hours of maturation;
• c) introducing a whole cell and/or genome of said cultured cells of step a) into said enucleated oocyte of step b) to obtain reconstructed embryos, wherein said enucleated oocyte of step a) is inactivated before and/or after introduction of said cell and/or genome of said cell into said oocyte;
• d) developing said reconstructed embryo of step c) to obtain an animal.