Diagnostic fluorescence and reflectance

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with a first and second electromagnetic wavelength, the first electromagnetic wavelength being selected from the range 330-350 nm and the second electromagnetic wavelength being selected from the range 370-450 nm;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with a first and second electromagnetic wavelength, the first electromagnetic wavelength being selected from the range 330-340 nm and the second electromagnetic wavelength being selected from the range 410-420 nm;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• de from the set of fluorescence intensity spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with a first and second electromagnetic wavelenth, the first electromagnetic wavelength being selected from the range 330-350 nm and the second electromagnetic wavelength being selected from the range 400-450 nm;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with a single electromagnetic wavelength, the single electromagnetic wavelength being selected from the range 370-400 nm;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with a first, second, and third electromagnetic wavelength, the first electromagnetic wavelength being selected from the range 330-340 nm, the second electromagnetic wavelength being selected from the range 350-380 nm, and the third electromagnetic wavelength being selected from the range 400-450 nm;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with an excitation light and a first, second, and third electromagnetic wavelength, the first electromagnetic wavelength being selected from the range 330-360 nm, the second electromagnetic wavelength being selected from the range 420-430 nm, and the third electromagnetic wavelength being selected from the range 460-470 nm;
• detecting, with the fiber optic probe, a set of reflectance spectra emitted from the tissue sample as a result of illumination with the excitation light, the fiber optic probe comprising at least one collection fiber positioned at at least one source-detector separation selected from the group consisting of 250 μm separation, 1.1 mm separation, 2.1 mm separation, and 3.0 mm separation;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra or the set of reflectance spectra, or a combination of the set of fluorescence intensity spectra and the set of reflectance spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with an excitation light and a first and second electromagnetic wavelength, the first electromagnetic wavelength being selected from the range 330-360 nm, and the second electromagnetic wavelength being 460 nm;
• detecting, with the fiber optic probe, a set of reflectance spectra emitted from the tissue sample as a result of illumination with the excitation light, the fiber optic probe comprising at least one collection fiber positioned at at least one source-detector separation selected from the group consisting of 250 μm separation, 1.1 mm separation, 2.1 mm separation, and 3.0 mm separation;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra or the set of reflectance spectra, or a combination of the set of fluorescence intensity spectra and the set of reflectance spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with an excitation light and a first and second set of electromagnetic wavelength, the first electromagnetic wavelength being selected from the range 330-350 nm and the second electromagnetic wavelength being 470 nm;
• detecting, with the fiber optic probe, a set of reflectance spectra emitted from the tissue sample as a result of illumination with the excitation light, the fiber optic probe comprising at least one collection fiber positioned at at least one source-detector separation selected from the group consisting of 250 μm separation, 1.1 mm separation, 2.1 mm separation, and 3.0 mm separation;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra or the set of reflectance spectra, or a combination of the set of fluorescence intensity spectra and the set of reflectance spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with an excitation light and a first and second electromagnetic wavelength, the first electromagnetic wavelength being selected from the range 330-350 nm and the second electromagnetic wavelength being selected from the range 470-480 nm;
• detecting, with the fiber optic probe, a set of reflectance spectra emitted from the tissue sample as a result of illumination with the excitation light, the fiber optic probe comprising at least one collection fiber positioned at at least one source-detector separation selected from the group consisting of 250 μm separation, 1.1 mm separation, 2.1 mm separation, and 3.0 mm separation;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra or the set of reflectance spectra, or a combination of the set of fluorescence intensity spectra and the set of reflectance spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with an excitation light and a first, second, and third electromagnetic wavelength, the first electromagnetic wavelength being selected from the range 350-360 nm, the second electromagnetic wavelength being selected from the range 420-430 nm, and the third electromagnetic wavelength being 460 nm;
• detecting, with the fiber optic probe, a set of reflectance spectra emitted from the tissue sample as a result of illumination with the excitation light, the fiber optic probe comprising at least one collection fiber positioned at at least one source-detector separation selected from the group consisting of 250 μm separation, 1.1 mm separation, 2.1 mm separation, and 3.0 mm separation;
• detecting the set of fluorescence intensity spectra emitted from the-tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra or the set of reflectance spectra, or a combination of the set of fluorescence intensity spectra and the set of reflectance spectra whether the tissue sample is normal or abnormal.

A method of detecting tissue abnormality in a tissue sample in vivo comprising:

• providing a tissue sample;
• sequentially illuminating the tissue sample in vivo with an excitation light and a first and second electromagnetic wavelength, the first electromagnetic wavelength being selected from the range 330-350 nm, the second electromagnetic wavelength being selected from the range 460-470 nm;
• detecting, with the fiber optic probe, a set of reflectance spectra emitted from the tissue sample as a result of illumination with the excitation light, the fiber optic probe comprising at least two collection fiber positioned at at least one source-detector separation selected from the group consisting of 250 μm separation, 1.1 mm separation, 2.1 mm separation, and 3.0 mm separation;
• detecting the set of fluorescence intensity spectra emitted from the tissue sample as a result of illumination;
• and
• determining from the set of fluorescence intensity spectra or the set of reflectance spectra, or a combination of the set of fluorescence intensity spectra and the set of reflectance spectra whether the tissue sample is normal or abnormal.