Murine alpha (1,3) fucosyltransferase Fuc-TVII, DNA encoding the same, method for preparing the same, antibodies recognizing the same, immunoassays for detecting the same, plasmids containing such DNA, and cells containing such a plasmid
AssigneeThe Regents of the University of Michigan
InventorsShunji Natsuka, Kevin M. Gersten, John B. Lowe
Life of this patent
Estimating the term…
Abstract
A gene which encodes a murine leukocyte α(1,3)fucosyltransferase capable of synthesizing the sialyl Lewis x determinant has been cloned.
Claims16
A polypeptide having an amino acid sequence which comprises an amino acid subsequence, said amino acid subsequence being selected from the group consisting of:
- (a) the amino acid sequence encoded by the DNA sequence corresponding to from position 966 to 1149 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2;
- and
- (b) the amino acid sequence encoded by the DNA sequence corresponding to from position 1947 to 1959 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2.
An isolated sequence of DNA which encodes a polypeptide having an amino acid sequence which comprises an amino acid subsequence, said amino acid subsequence being selected from the group consisting of:
- (a) the amino acid sequence encoded by the DNA sequence corresponding to from position 966 to 1149 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2;
- and
- (b) the amino acid sequence encoded by the DNA sequence corresponding to from position 1947 to 1959 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2.
A plasmid, comprising a sequence of DNA which encodes a polypeptide having an amino acid sequence which comprises an amino acid subsequence, said amino acid subsequence being selected from the group consisting of:
- (a) the amino acid sequence encoded by the DNA sequence corresponding to from position 996 to 1149 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2;
- and
- (b) the amino acid sequence encoded by the DNA sequence corresponding to from position 1947 to 1959 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2.
A transformed cell, which comprises a plasmid comprising a sequence of DNA which encodes a polypeptide having an amino acid sequence which comprises an amino acid subsequence, said amino acid subsequence being selected from the group consisting of:
- (a) the amino acid sequence encoded by the DNA sequence corresponding to from position 996 to 1149 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2;
- and
- (b) the amino acid sequence encoded by the DNA sequence corresponding to from position 1947 to 1959 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2.
An antibody which binds specifically to a polypeptide having an amino acid sequence which comprises an amino acid subsequence, said amino acid subsequence being selected from the group consisting of:
- (a) the amino acid sequence encoded by the DNA sequence corresponding to from position 996 to 1149 and 0.2067 to 3079 of the nucleotide sequence shown in FIG. 2;
- and
- (b) the amino acid sequence encoded by the DNA sequence corresponding to from position 1947 to 1959 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2.
An immunoassay for a polypeptide, comprising
- (i) contacting a sample which may contain said polypeptide with an antibody which specifically binds to said polypeptide to form an antibody-polypeptide complex;
- and
- (ii) detecting said antibody-polypeptide complex;
- wherein said polypeptide has an amino acid sequence which comprises an amino acid subsequence, said amino acid subsequence being selected from the group consisting of:
- (a) the amino acid sequence encoded by the DNA sequence corresponding to from position 996 to 1149 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2;
- and
- (b) the amino acid sequence encoded by the DNA sequence corresponding to from position 1947 to 1959 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2.
An isolated sequence of DNA, which comprises a DNA subsequence corresponding to nucleotide positions 966 to 3079 of the DNA sequence shown in FIG. 2.
An isolated sequence of DNA, which comprises a DNA subsequence corresponding to nucleotide positions 1947 to 3079 of the DNA sequence shown in FIG. 2.
A method for producing a polypeptide, comprising culturing a transformed cell, which comprises a plasmid comprising a sequence of DNA which encodes a polypeptide having an amino acid sequence which comprises an amino acid subsequence, said amino acid subsequence being selected from the group consisting of:
- (a) the amino acid sequence encoded by the DNA sequence corresponding to from position 996 to 1149 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2;
- and
- (b) the amino acid sequence encoded by the DNA sequence corresponding to from position 1947 to 1959 and 2067 to 3079 of the nucleotide sequence shown in FIG. 2.
Dependencies are inferred from the claim text.