Cell surface display of proteins by recombinant host cells
AssigneeZymoGenetics, Inc.
InventorSi Lok
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Abstract
Methods and vectors are described for expressing recombinant proteins on the surface of host cells. These processes and compositions provide the basis for strategies to produce a fusion protein, comprising a membrane anchor that allows extracellular attachment of the fusion protein in a type II orientation.
Claims29
An isolated nucleic acid molecule, comprising (a) a eukaryotic promoter, (b) a nucleotide sequence encoding a type II signal anchor domain segment, and (c) a cloning site, wherein the isolated nucleic acid molecule comprises elements (a) to (c) in a 5′ to 3′ order.
10 An expression vector, comprising the isolated nucleic acid molecule of claim 1.
An isolated nucleic acid molecule, comprising (a) a eukaryotic promoter, (b) a nucleotide sequence encoding a type II signal anchor domain segment, and (c) a gene or gene fragment, wherein the isolated nucleic acid molecule comprises elements (a) to (c) in a 5′ to 3′ order, wherein the gene or gene fragment resides in-frame with the nucleotide sequence that encodes the type I signal anchor domain, and wherein the nucleotide sequence that encodes a type II signal anchor domain segment is heterologous with respect to the gene or gene fragment.
An isolated nucleic acid molecule, consisting of (a) a eukaryotic promoter, (b) a nucleotide sequence encoding a type It signal anchor domain segment, and (c) a gene or gene fragment, wherein the isolated nucleic acid molecule comprises elements (a) to (c) in a 5′ to 3′ order, wherein the gene or gene fragment resides in-frame with the nucleotide sequence that encodes the type II signal anchor domain, and wherein the nucleotide sequence that encodes a type II signal anchor domain segment is heterologous with respect to the gene or gene fragment.
A kit comprising the expression vector of claim 10.
Dependencies are inferred from the claim text.