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US 2004/0063134 A1Published application
Novel isoforms of human pregnancy-associated protein-E
OwnerYizhong Gu, Mark E. Shannoninventors
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OwnerYizhong Gu, Mark E. Shannoninventors
Estimating the term…
The invention provides isolated nucleic acids that encode three novel isoforms of human pregnancy associated plasma protein E, hPAPP-E, and fragments thereof, vectors for propagating and expressing PAPP-E nucleic acids, host cells comprising the nucleic acids and vectors of the present invention, proteins, protein fragments, and protein fusions of the novel PAPP-E isoforms, and antibodies thereto. The invention further provides transgenic cells and non-human organisms comprising human PAPP-E isoform nucleic acids, and transgenic cells and non-human organisms with targeted disruption of the endogenous orthologue of the human PAPP-E gene. The invention further provides pharmaceutical formulations of the nucleic acids, proteins, and antibodies of the present invention, and diagnostic, investigational, and therapeutic methods based on the PAPP-E nucleic acids, proteins, and antibodies of the present invention.
An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
An isolated nucleic acid comprising a sequence that hybridizes under high stringency conditions to a hybridization probe the nucleotide sequence of which (i) encodes a polypeptide having the sequence of SEQ ID NO:7, (ii) encodes a polypeptide having the sequence of SEQ ID NO:7 with conservative amino acid substitutions, or (iii) is the complement of (i) or (ii),
An isolated nucleic acid comprising at least 17 nucleotides of (i) SEQ ID NO:4 or (ii) the complement of SEQ ID NO:4,
An isolated nucleic acid comprising (i) a nucleotide sequence that encodes a peptide of at least 8 contiguous amino acids of SEQ ID NO:7, or (ii) the complement of a nucleotide sequence that encodes a peptide of at least 8 contiguous amino acids of SEQ ID NO:7,
An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
An isolated nucleic acid comprising a nucleotide sequence (i) that encodes a polypeptide having the sequence of SEQ ID NO:14, (ii) that encodes a polypeptide having the sequence of SEQ ID NO:14 with conservative amino acid substitutions, or (iii) the complement of a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO:14,
An isolated nucleic acid comprising a sequence that hybridizes under high stringency conditions to a probe the nucleotide sequence of which consists of SEQ ID NO:11 or the complement of SEQ ID NO:11,
An isolated nucleic acid comprising at least 17 nucleotides of (i) SEQ ID NO:11, (ii) a degenerate variant of SEQ ID NO:11, or (iii) the complement of (i) or (ii),
An isolated nucleic acid comprising a nucleotide sequence that (i) encodes a polypeptide having the sequence of at least 8 contiguous amino acids of SEQ ID NO:12, (ii) encodes a polypeptide having the sequence of at least 8 contiguous amino acids of SEQ ID NO:12 with conservative amino acid substitutions, or (iii) is the complement of (i) or (ii).
An isolated nucleic acid comprising a nucleotide sequence at least 90% identical to SEQ ID NO:11.
An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
An isolated nucleic acid comprising a sequence selected from the group consisting of:
An isolated nucleic acid comprising a nucleotide sequence of no more than one fragment of SEQ ID NOs:19 to 41 or the complement of SEQ ID NOs:
The isolated nucleic acid of claim 68, wherein said fragment comprises at least 50 contiguous nucleotides of said SEQ ID NO:
An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:65 or its complement,
A microarray, comprising:
An isolated polypeptide, said polypeptide comprising the amino acid sequence of SEQ ID NO:3, SEQ ID NO:10, or SEQ ID NO:16.
An isolated polypeptide comprising a sequence of at least 8 contiguous amino acids of SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, or SEQ ID NO:18.
An antibody, said antibody selected from the group consisting of:
A method of antenatal diagnosis of dysgenetic pregnancies, comprising:
Dependencies are inferred from the claim text.