Prism-based flow cytometry excitation optics

Abstract

A prism-based cytometry excitation optics system redirects, shapes and combines plural beams provided by light sources, so they can be focused by an objective into closely spaced spots within the channel of a cytometry flow cell. The mounted set of dispersive prisms redirect beams of different wavelengths so that beams with separated inputs emerge from the final prism, substantially overlapping in position and nearly parallel to one another. Preferred embodiments having a mounted set of four distinct isosceles triangular prisms are described with particular orientations and positions, but the specific design parameters may vary depending on the choice of beam wavelengths, spot separations in the flow cell, and choice of prism material. The prism system has inherently better thermal and long-term stability compared to mirror-based optics.

Dependencies are inferred from the claim text.