Production of chimeric human papillomavirus
AssigneeMerck & Co., Inc.
InventorsRobert S. Lowe, Craig M. Meyers, Jiaping Zhang, Michelle Kaupas, Kathrin Ute Jansen
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Abstract
This invention relates to infectious chimeric papillomaviruses, and especially those where the early genes are from human papillomavirus (HPV) 18, and the late genes are from another HPV. Also presented are methods of culturing the virus in raft cell cultures, and to assays utilizing these chimeric viruses.
Claims14
An infectious chimeric papillomavirus.
A vector encoding a chimeric infectious papillomavirus.
A host cell comprising a chimeric infectious papillomavirus.
A method of obtaining an infectious chimeric papillomavirus comprising:
- (a) preparing a chimeric papillomavirus wherein early genes are from a first papillomavirus source and late genes are from a second papillomavirus source;
- (b) preparing a raft cell culture of differentiating epithelial cells;
- (c) infecting the cell culture with the chimeric papillomavirus;
- and
- (d) incubating the cell culture under conditions which support viral replication and
- (e) harvesting the resultant chimeric papillomavirus.
An assays to detect the presence of a human papillomavirus neutralizing antibody in a serum comprising:
- (a) contacting a cell culture comprising infectious chimeric papillomavirus with the serum;
- and
- (b) determining if chimeric papillomavirus replication occurs with in the cell culture in the presence of the serum.
Dependencies are inferred from the claim text.