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AssigneeHer Majesty the Queen in right of Canada, as represented by the Minister of Natural Resources Canada, Canadian Forest Service
InventorsAbul M. K. Ekramoddoullah, Jun-Jun Liu
Estimating the term…
There is provided an isolated polynucleotide sequence useful as a promoter in plant root tissue. The sequence is derived from the promoter for the Western white pine gene PR10. The sequence comprises at least 45 nucleotidcs, and has at least two Dof elements which individually may be in a forward or inverted orientation. Each Dof element has a sequence NNNWAAAGNNN, wherein N is A, T, G, or, C, and, W is A or T. The Dof elements are separated from each other by between 10 and 20 nucleotides. The sequence also has a TATA box located between 15 and 25 nucleotides in the 3′ direction of the closest Dof element. In some instances, the polynucleotide has one Dof element with the sequence NNNWAAAGNNN and another with the sequence NNNCTTTWNNN. In some instances the polynucleotide sequence is one where the 5′ Dof element sequence is CTCTCTTTAAT, and another Dof element (which may be the next Dof element) sequence is TTTAAAGGTT and the TATA box sequence is TACAATAAATA or TATAWAWA, wherein W is A or T. Also provided are sequences useful in modulating gene expression in stem and leaf tissue.
An isolated polynucleotide sequence comprising SEQ.ID.NO. 2 or a variant at least 70% homologous thereto.
An isolated polynucleotide sequence, said sequence comprising at least 45 nucleotides, said sequence having at least two Dof elements which individually may be in a forward or inverted orientation;
An isolated polynucleotide sequence useful in reducing expression Of a downstream gene, said polynucleotide sequence comprising two copies of a DNA region at least 70% homologous to a portion of substantially equal length located between nucleotides 361 and 639 of SEQ.ID.NO.1, said copies being separated by between 0 and 100 nucleotides.
An isolated polynucleotide sequence useful in increasing expression of a downstream gene, said polynucleotide containing a region of between 100 and 225 nucleotides, said region being at least 70% homologous to a portion of SEQ.ID.NO.20 located between nucleotides 642 and 867 and being of substantially the same length as said region.
A method of obtaining expression of a gene of interest in root tissue, comprising:
An isolated polynucleotide sequence comprising a region of at least 100 contiguous nucleotides, said region being at least 70% homologous to a 100 bp region SEQ.ID.NO.21 or SEQ.ID.NO.22.
Dependencies are inferred from the claim text.