Plant retroelements and methods related thereto
AssigneeIowa State University Research Foundation, Inc.
InventorsDavid A. Wright, Daniel F. Voytas
Life of this patent
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Abstract
The present invention provides nucleic acids, as well as vectors, cells, and plants (including plant parts, seeds, and embryos) containing the nucleic acids. In particular, molecular tools are provided in the form of nucleic acids that encode reverse transcriptases. The invention also features methods for manipulating such nucleic acids. In addition, the invention features methods to introduce nucleic acids containing retroelements or retroelement sequences into cells.
Claims14
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) a sequence having at least 60% identity to a nucleotide sequence of one or more of the even-numbered SEQ ID NOs from SEQ ID NO:42 to SEQ ID NO:
- 164;
- (b) a sequence encoding a polypeptide with an amino acid sequence having at least 60% identity to an amino acid sequence of one or more of the odd-numbered SEQ ID NOs from SEQ ID NO:43 to SEQ ID NO:165;
- (c) a sequence that is an allelic variant of (a) or (b);
- and
- (d) a sequence that is fully complementary to a nucleotide sequence of (a), (b), or (c).
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a reverse transcriptase, said reverse transcriptase comprising any two of the following amino acid motifs:
- (a) Trp-Val-Ser within reverse transcriptase domain 1;
- (b) Thr-Val/Ile-Val/Ile-Xaa-Xaa-Xaa-Xaa-Xaa-Asp/Glu-Leu-Val/Ile (SEQ ID NO:191) between reverse transcriptase domains 1 and 2;
- (c) Thr/Ser-Arg/Lys-Arg/Lys-Asp-His (SEQ ID NO:
- 192) within reverse transcriptase domain 2;
- (d) Met-Leu-Asp/Glu-Arg-Leu (SEQ ID NO:
- 193) spanning the boundary between reverse transcriptase domains 2 and 3;
- (e) Cys-Phe-Leu-Asp-Gly-Tyr-Ser (SEQ ID NO:
- 194) within reverse transcriptase domain 3;
- (f) Phe-Thr-Cys-Pro (SEQ ID NO:195) within reverse transcriptase domain 3;
- (g) Phe-Gly-Leu-Cys-Asn-Ala-Pro (SEQ ID NO:
- 196) within reverse transcriptase domain 4;
- (h) Phe-Met-Asp-Asp-Phe (SEQ ID NO:197) within reverse transcriptase domain 5;
- (i) Leu-Val/Ile-Leu-Asn-Trp-Glu-Lys-Cys-His-Phe-Met-Val/Ile (SEQ ID NO:198) spanning the boundary between reverse transcriptase domains 6 and 7;
- and
- (j) Gly-Leu/Ile-Val-Leu-Gly-His (SEQ ID NO:200) within reverse transcriptase domain 7.
11 The stably transformed cell of claim 8, wherein said cell is a prokaryotic cell.
An Athila retrotransposon comprising an open reading frame that has no substantial sequence similarity to retrotransposon gag or pol open reading frames, wherein said open reading frame is positioned downstream from an Athila integrase open reading frame.
Dependencies are inferred from the claim text.