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AssigneesAviGenics, Inc., University of Georgia Research Foundation, Inc.
InventorsRobert D. Ivarie, Alex J. Harvey, Julie A. Morris, Guodong Liu, Jeffrey C. Rapp
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This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of avians in order to express the exogenous sequences to alter the phenotype of the avians or to produce desired proteins. In particular, transgenic avians are produced which express exogenous sequences in their oviducts and which deposit exogenous proteins into their eggs. Avian eggs that contain exogenous proteins are encompassed by this invention. The instant invention further provides novel forms of interferon and erythropoietin which are efficiently expressed in the oviduct of transgenic avians and deposited into avian eggs.
A vector comprising a coding sequence and a promoter in operational and positional relationship to express said coding sequence in an avian oviduct, wherein said coding sequence is selected from the group consisting of recombinant transgenic poultry derived interferon-α 2b and erythropoietin coding sequence.
A transgenic avian having a transgene in the genetic material of its germ-line tissue, wherein the transgene comprises an exogenous gene and a promoter in operational and positional relationship to express transgenic poultry derived interferon-α 2b or erythropoietin, wherein said exogenous gene is expressed in an avian oviduct of said transgenic avian.
An egg of an avian species containing protein exogenous to the avian species, wherein said protein is selected from the group consisting of transgenic poultry derived interferon-α 2b and erythropoietin.
A method for producing transgenic poultry derived interferon-α 2b or erythropoietin protein in an avian oviduct, comprising:
A method for producing an avian egg which contains transgenic poultry derived interferon-α 2b or erythropoietin protein, comprising:
An isolated polynucleotide comprising the optimized polynucleotide sequence of human interferon-α 2b (SEQ ID NO:
An isolated protein comprising the polypeptide sequence of transgenic poultry derived interferon-α 2b, wherein said protein is O-glycosylated at Thr-106 with
A pharmaceutical composition comprising the polypeptide sequence of transgenic poultry derived interferon-α 2b, wherein said protein is O-glycosylated at Thr-106 with
An isolated polynucleotide comprising the optimized polynucleotide sequence of human erythropoietin (SEQ ID NO:
An MDOT promoter, comprised of elements from the ovomucoid (MD) and ovotransferrin (OT) promoters.
A vector comprising a first and second coding sequence and a promoter in operational and positional relationship to said first and second coding sequence to express said first and second coding sequence in an avian oviduct;
Dependencies are inferred from the claim text.