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US 2004/0019192 A1Published application
Process for modifying plants
OwnerMark Harker, Susan Amanda Hellyer +2inventors
InventorsMark Harker, Susan Amanda Hellyer, Niklas Holmberg, Dick Safford
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Abstract
The use of a gene expressing a non-feed back inhibited HMG-reductase in combination with a gene expressing sterol methyltransferasel to increase the level of sterols in plants.
Claims16
The use of a gene expressing a non-feed back inhibited HMG-reductase in combination with a gene expressing sterol methyltransferasel to increase the level of sterols in plants.
A method of transforming a plant by
- A1) transforming a plant cell with a recombinant DNA construct comprising a DNA segment encoding a polypeptide with non feedback inhibited HMGR activity and a polypeptide encoding a sterol methyltransferasel activity and promoters for driving the expression of said polypeptides in said plant cell to form a transformed plant cell;
- or
- A2) re-transforming a plant cell expressing a non-feedback inhibited HMGR activity with a gene encoding a sterol methyltransferasel activity;
- or
- A3) re-transforming a plant cell expressing a sterol methyltransferasel activity with a gene encoding a non-feedback inhibited HMGR activity;
- and
- D) regenerating the above transformed plant cells into transgenic plants;
- and
- E) selecting transgenic plants that have enhanced levels of 4-desmethylsterols compared to wild type strains of the same plant.
6 more elements
Plant having incorporated in its genome a heterologous gene encoding a non-feed back inhibited HMGR activity in combination with an heterologous gene encoding SMT1.
Dependencies are inferred from the claim text.