Terminal labeled probe array and method of making it

A method for evaluating an amount of a target substance comprising the steps of:

• reacting a probe array and a target substance wherein the probe array comprises a plurality of probes immobilized at a plurality of matrix sites on a substrate for capturing a target substance, the probes are sequentially synthesized at the matrix sites on the substrate until a desired length, the probes are different from each other, and a labeling compound is coupled to each terminus of the probes in a final step of the synthesis;
• measuring an amount of the labeling compound at each matrix site to determine an amount of the probe at the matrix site;
• measuring an amount of a labeled target substance captured by the probe at the matrix site;
• and
• comparing the amount of the probe with the amount of the labeled target substance.

A method of evaluating an amount of a target substance comprising the steps of:

• reacting a probe array and a target substance, wherein the probe array comprises a plurality of probes immobilized at a plurality of matrix sites on a substrate for capturing a target substance, the probes are sequentially synthesized at the matrix sites on the substrate until a desired length, the probes are different from each other, and a labeling compound is coupled to each terminus of the probes in a final step of the synthesis;
• measuring an amount of the labeling compound at each matrix site to determine an amount of the probe at the matrix site;
• measuring an amount of a labeled target substance captured by the probe at the matrix site;
• measuring an amount of the labeling compound directly bonded to the substrate at a predetermined matrix site on the surface of the substrate during a first step of the sequential synthesis without elongation reaction;
• and
• comparing the amount of the probe, the amount of the labeled target substance, and the amount of the directly bonded labeling compound.