Starch R1 phosphorylation protein homologs
OwnerStephen M. Allen, Karen E. Broglie +2inventors
InventorsStephen M. Allen, Karen E. Broglie, Karlene H. Butler, Robert F. Cressman
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a starch R1 phosphorylation protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the starch R1 phosphorylation protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the starch R1 phosphorylation protein in a transformed host cell.
Claims19
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having starch R1 phosphorylation activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:
- 18 have at least 85% sequence identity based on the Clustal alignment method, or
- (b) a full-length complement of the nucleotide sequence of (a).
An isolated polypeptide having starch R1 phosphorylation activity, wherein the polypeptide has an amino acid sequence of at least 85% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:
- 18.
An isolated polynucleotide comprising:
- (a) a first nucleotide sequence encoding a first polypeptide comprising at least 600 amino acids, wherein the amino acid sequence of the first polypeptide and the amino acid sequence of SEQ ID NO:20 have at least 80% identity based on the Clustal alignment method,
- (b) a second nucleotide sequence encoding a second polypeptide comprising at least 1337 amino acids, wherein the amino acid sequence of the second polypeptide and the amino acid sequence of SEQ ID NO:
- 16 or SEQ ID NO:
- 18 have at least 80% identity based on the Clustal alignment method, or
- (c) the complement of the first or second nucleotide sequence, wherein the complement and the first or second nucleotide sequence contain the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.