Methods of diagnosing cervical cancer
AssigneeArbor Vita Corporation
InventorsPeter S. Lu, Johannes Schweizer, Chamorro Somoza Diaz-Sarmiento, Michael P. Belmares
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Abstract
The invention provides reagents and methods for detecting pathogen infections in human samples. This detection utilizes specific proteins to detect the presence of pathogen proteins or abnormal expression of human proteins resulting from pathogen infections. Specific methods, compositions and kits are disclosed herein for the detection of oncogenic Human papillomavirus E6 proteins in clinical samples.
Claims20
A method of detecting the presence of an oncogenic HPV E6 protein in a sample, said method comprising:
- contacting a sample suspected of containing an oncogenic HPV E6 protein with a PDZ domain polypeptide;
- and
- detecting any binding of said oncogenic HPV E6 protein in said sample to said PDZ domain polypeptide;
- wherein binding of said oncogenic HPV E6 protein to said PDZ domain polypeptide indicates the presence of an oncogenic HPV E6 protein in said sample.
A system for detecting the presence of an oncogenic HPV E6 polypeptide in a sample, said method comprising:
- a first and a second binding partner for an oncogenic HPV E6 polypeptide,
- wherein said first binding partner is a PDZ domain protein and at least one of said binding partners is attached to a solid support.
A method for determining if a subject is infected with an oncogenic strain of HPV, said method comprising:
- detecting the presence of oncogenic HPV E6 protein in a sample from said subject using an oncogenic HPV E6 protein-binding PDZ protein,
- wherein the presence of oncogenic HPV E6 protein indicates that the subject is infected with an oncogenic strain of HPV.
A kit for testing for the presence of oncogenic HPV E6 protein, the kit comprising first and second binding partners for said oncogenic HPV E6 protein, wherein said first binding partner is a PDZ domain protein.
Dependencies are inferred from the claim text.