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US 2004/0006395 A1Published application
Vascularization enhanced graft constructs
AssigneePurdue Research Foundation
InventorStephen F. Badylak
Life of this patent
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AssigneePurdue Research Foundation
InventorStephen F. Badylak
Estimating the term…
A tissue graft construct for use in repairing diseased or damaged tissues is provided. The tissue graft construct comprises a matrix composition selected from the group consisting of urinary bladder submucosa and stomach submucosa, and extracts and hydrolysates thereof, added endothelial cells, and at least one additional preselected, exogenous population of cells which enhance initiation of the formation vessel-like structures in the graft construct. The preselected population of cells can be a population of non-keratinized or keratinized epithelial cells or a population of mesodermally-derived cells selected from the group consisting of fibroblasts, smooth muscle cells, skeletal muscle cells, cardiac muscle cells, multi-potential progenitor cells, pericytes, osteogenic cells, and any other suitable cell type, preferably selected based on the tissue to be repaired. Methods for enhancing the vascularization in vivo of these tissue graft constructs and for preparing these graft constructs are also provided.
A tissue graft construct for use in repairing diseased or damaged tissues, said tissue graft construct comprising a matrix composition selected from the group consisting of urinary bladder submucosa and stomach submucosa, and extracts and hydrolysates thereof, added endothelial cells, and at least one additional preselected, exogenous population of cells.
A method for enhancing the vascularization in vivo of a tissue graft construct, said method comprising the steps of:
A method of preparing a tissue graft construct for use in repairing diseased or damaged tissues, said method comprising the step of seeding in vitro a matrix composition selected from the group consisting of urinary bladder submucosa and stomach submucosa, and extracts and hydrolysates thereof, with a population of endothelial cells, and at least one additional preselected, exogenous population of cells.
A method for enhancing the vascularization in vivo of a graft construct said method comprising the steps of:
A vascularized tissue graft construct for use in repairing diseased or damaged tissues, said tissue graft construct comprising a matrix composition selected from the group consisting of urinary bladder submucosa and stomach submucosa, and extracts and hydrolysates thereof, added endothelial cells, and at least one additional preselected, exogenous population of cells wherein said endothelial cells have been cultured on said matrix composition for a time sufficient to form vessels or vessel-like structures in vitro.
A tissue graft construct having vessel-like structures for use in repairing diseased or damaged tissues, said tissue graft construct comprising a matrix composition selected from the group consisting of urinary bladder submucosa and stomach submucosa, and extracts and hydrolysates thereof, added endothelial cells, and at least one additional preselected, exogenous population of cells wherein said additional population of cells enhances the initiation of formation of said vessel-like structures in said graft construct.
A method of preparing a tissue graft construct having vessel-like structures for use in repairing diseased or damaged tissues, said method comprising the step of seeding in vitro a matrix composition selected from the group consisting of urinary bladder submucosa and stomach submucosa, and extracts and hydrolysates thereof, with a population of endothelial cells, and at least one additional preselected, exogenous population of cells wherein said additional population of cells enhances the initiation of formation of said vessel-like structures in said graft construct.
Dependencies are inferred from the claim text.