Cellulose degrading enzymes of aspergillus
OwnerMarcus Matheus Catharina Gielkens, Jacob Visser +1inventors
InventorsMarcus Matheus Catharina Gielkens, Jacob Visser, Leendert Hendrik De Graaff
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Abstract
Two cellobiohydrolase polypeptides (CBHA and CBHB) derived from Aspergillus are described and can be used to degrade cellulose. Variants of these peptides are described as well as DNA encoding the peptides, vectors and host cells. The peptides can be used to produce or process food, animal feed, wood pulp, paper and textiles.
Claims25
A polypeptide which comprises the sequence of SEQ ID No:
- 8 (CBH A) or SEQ ID No:10 (CBH B), a sequence substantially homologous to either sequence, or a fragment of any of these sequences.
A polypeptide from Aspergillus which is a CBH or has CBH activity.
A polypeptide which:
- (i) has CBH activity;
- (ii) has an activity of at least 50% of the maximum activity over the pH range from 3 to 5;
- (iii) has an optimum activity at a temperature is greater than 50° C.;
- and optionally
- (iv) does not have a cellulose binding domain or a linker peptide.
A polypeptide according to any one of the preceding claims which has CBHI activity.
A polypeptide according to any of the preceding claims which is obtainable from or produced by a fungus.
6 A polypeptide according to claim 4 which is obtainable from or produced by Aspergillus.
A polynucleotide comprising a sequence encoding a polypeptide according to any one of the preceding claims.
A polynucleotide capable of selectively hybridising to SEQ ID No:
- 5 or SEQ ID No:9 or a complement thereof.
Dependencies are inferred from the claim text.