Materials and methods for the modification of plant cell wall polysaccharides
AssigneesGenesis Research & Development Corporation Limited, Rubicon Forests Holdings Limited
InventorLeonard N. Bloksberg
Life of this patent
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Abstract
Novel isolated polynucleotides and polypeptides associated with the synthesis of plant cell wall polysaccharides are provided, together with genetic constructs comprising such sequences. Methods for using such constructs for the modulation of polysaccharide content in plants are also disclosed, together with transgenic plants comprising such constructs.
Claims21
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- SEQ ID NOS:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955.
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) complements of the sequences recited in SEQ ID NOS:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955;
- (b) reverse complements of the sequences recited in SEQ ID NOS:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955;
- and
- (c) reverse sequences of the sequences recited in SEQ ID NOS:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955;
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) nucleotide sequences having at least 75% identity to a nucleotide sequence recited in SEQ ID NOS:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955;
- (b) nucleotide sequences having at least 90% identity to a nucleotide sequence recited in SEQ ID NOS:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955;
- and
- (c) nucleotide sequences having at least 95% identity to a nucleotide sequence recited in SEQ ID NOS:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955,
- wherein the polynucleotide encodes a polypeptide having substantially the same functional properties as a polypeptide encoded by SEQ ID NO:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955.
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) nucleotide sequences that hybridize to a sequence recited in SEQ ID NO:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955 under stringent hybridization conditions;
- (b) nucleotide sequences that are 200-mers of a sequence recited in SEQ ID NO:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955;
- (c) nucleotide sequences that are 100-mers of a sequence recited in SEQ ID NO:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955;
- (d) nucleotide sequences that are 40-mers of a sequence recited in SEQ ID NO:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955;
- (e) nucleotide sequences that are 20-mers of a sequence recited in SEQ ID NO:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955;
- and
- (f) nucleotide sequences that are degeneratively equivalent to a sequence recited in SEQ ID NO:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955.
An oligonucleotide probe or primer comprising at least 10 contiguous residues complementary to 10 contiguous residues of a nucleotide sequence recited in SEQ ID NO:
- 1-29, 57-80, 105, 107, 109-113, 119-128, 139-143, 149-933 and 955.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- SEQ ID NOS:
- 30-56, 81-104, 106, 108, 114-118, 129-138, 144-148, 934-954 and 956.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) sequences having at least 70% identity to a sequence of SEQ ID NOS:
- 30-56, 81-104, 106, 108, 114-118, 129-138, 144-148,934-954 and 956;
- (b) sequences having at least 90% identity to a sequence of SEQ ID NOS:
- 30-56, 81-104, 106, 108, 114-118, 129-138, 144-148, 934-954 and 956;
- and
- (c) sequences having at least 95% identity to a sequence of SEQ ID NOS:
- 30-56, 81-104, 106, 108, 114-118, 129-138, 144-148,934-954 and 956,
- wherein the polypeptide has substantially the same functional properties as a polypeptide of SEQ ID NOS:
- 30-56, 81-104, 106, 108, 114-118, 129-138, 144-148, 934-954 and 956.
Dependencies are inferred from the claim text.