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AssigneeThe Texas A&M University System
InventorsMark E. Westhusin, TaeYoung Shin, Jane Pryor, James Rugila, D. C. Kraemer
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This invention is the first cat cloned by nuclear transfer, as well as any subsequent progeny produced by regular sexual reproduction. Nuclear transfer methods and techniques involving feline somatic cells, nuclei, or nuclear DNA, and the products thereof, are disclosed. In a single experiment 3 cloned embryos derived from the cumulus cell line and 2 embryos derived from fibroblast cells were transferred into a recipient female cat. Following embryo transfer after the period of gestation, a kitten was delivered. The cloned kitten has the exact DNA fingerprint as the cumulus cell line of the adult female cat from which the cumulus cell line was derived.
A method of cloning a feline comprising:
A method of cloning a cat comprising:
The method of claim, 39, wherein the activated cybrid is incubated in the fourth and fifth media for a total of up to 7 days prior to transfer into the reproductive tract of the second female cat.
A feline whose nuclear genome is identical to a single parent, wherein the parent is multicellular.
A feline whose nuclear genome is identical to a single adult cell.
A feline whose nuclear genome is identical to a single, multicellular parent produced from a method comprising:
A progeny feline whose nuclear genome is identical to a single ancestor.
Dependencies are inferred from the claim text.