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AssigneeArcher Daniels Midland Company
InventorsEui-Sung Choi, John D'Elia, Hye-Sun Kim, Mi-Soo Kim, Jung Kee Lee, Jae-Gu Pan, Steven F. Stoddard, Do-Young Yum
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The invention relates to the fields of molecular biology, bacteriology and industrial fermentation. More specifically, the invention relates to the identification and isolation of nucleic acid sequences and proteins of sorbitol dehydrogenases and cytochrome c of the strains, Ketogulonigenium spp. The invention further relates to the fermentative production of L-sorbose from D-sorbitol and the subsequent production of 2-keto-L-gulonic acid.
An isolated nucleic acid molecule comprising a polynucleotide sequence encoding a fragment at least about 10 amino acids in length of the amino acid sequence of SEQ ID NO:5.
An isolated nucleic acid molecule comprising a polynucleotide sequence comprising a fragment at least about 30 nucleotides in length of SEQ ID NO:1.
An isolated nucleic acid molecule comprising a polynucleotide sequence at least about 95% identical to SEQ ID NO:1.
A vector comprising a nucleic acid molecule which comprises a polynucleotide sequence selected from the group consisting of:
An isolated nucleic acid molecule comprising a polynucleotide sequence encoding a fragment at least about 10 amino acids in length of the amino acid sequence of SEQ ID NO:6.
An isolated nucleic acid molecule comprising a polynucleotide sequence comprising a fragment at least about 30 nucleotides in length of SEQ ID NO:2.
An isolated nucleic acid molecule comprising a polynucleotide sequence at least about 95% identical to SEQ ID NO:2.
A vector comprising a nucleic acid molecule which comprises a polynucleotide sequence selected from the group consisting of:
An isolated nucleic acid molecule comprising a polynucleotide sequence encoding a fragment at least about 10 amino acids in length of the amino acid sequence of SEQ ID NO:7.
An isolated nucleic acid molecule comprising a polynucleotide sequence comprising a fragment at least about 30 nucleotides in length of SEQ ID NO:3.
An isolated nucleic acid molecule comprising a polynucleotide sequence at least about 95% identical to SEQ ID NO:3.
A vector comprising a nucleic acid molecule which comprises a polynucleotide sequence selected from the group consisting of:
An isolated nucleic acid molecule comprising a polynucleotide sequence encoding a fragment at least about 10 amino acids in length of the amino acid sequence of SEQ ID NO:8.
An isolated nucleic acid molecule comprising a polynucleotide sequence comprising a fragment at least about 30 nucleotides in length of SEQ ID NO:4.
An isolated nucleic acid molecule comprising a polynucleotide sequence at least about 95% identical to SEQ ID NO:4.
A vector comprising a nucleic acid molecule which comprises a polynucleotide sequence selected from the group consisting of:
An isolated nucleic acid molecule comprising a polynucleotide sequence comprising a fragment at least about 20 nucleotides in length of the polynucleotide sequence of SEQ ID NO:9.
An isolated nucleic acid molecule comprising a polynucleotide sequence at least about 95% identical SEQ ID NO:9.
A vector comprising a nucleic acid molecule which comprises a polynucleotide sequence selected from the group consisting of:
An isolated nucleic acid molecule comprising a polynucleotide sequence comprising a fragment at least about 20 nucleotides in length of the polynucleotide sequence of SEQ ID NO:10.
An isolated nucleic acid molecule comprising a polynucleotide sequence at least about 95% identical SEQ ID NO:10.
A vector comprising a nucleic acid molecule which comprises a polynucleotide sequence selected from the group consisting of:
An isolated nucleic acid molecule comprising a polynucleotide sequence comprising a fragment at least about 20 nucleotides in length of the polynucleotide of SEQ ID NO:11.
An isolated nucleic acid molecule comprising a polynucleotide sequence at least about 95% identical SEQ ID NO:11.
A vector comprising a nucleic acid molecule which comprises a polynucleotide sequence selected from the group consisting of:
A process for the production of L-sorbose from D-sorbitol comprising:
A process for increasing the production of 2-keto-L-gulonic acid comprising:
An isolated polypeptide comprising a polypeptide sequence at least about 10 amino acids in length of the amino acid sequence of SEQ ID NO:5.
An isolated polypeptide comprising a polypeptide sequence at least about 10 amino acids of the polypeptide sequence of SEQ ID NO:6.
An isolated polypeptide comprising a polypeptide sequence at least about 10 amino acids of the polypeptide sequence of SEQ ID NO:7.
An isolated polypeptide comprising a polypeptide sequence at least about 10 amino acids of the polypeptide sequence of SEQ ID NO:8.
Dependencies are inferred from the claim text.