Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
OwnerDon Strausinventor
Life of this patent
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Abstract
The invention provides a method, referred to as genomic profiling, which simultaneously scans a complex biological sample for the presence of nucleic acid sequences (including genomic difference sequences, group-specific sequences, and DNA polymorphisms) that are diagnostic of numerous different types of organisms. Also included in the invention are probes, detection ensembles, and related molecules for use in the methods of the invention.
Claims57
A method for obtaining genetic information from a biological sample potentially comprising target nucleic acid molecules said method comprising the steps of:
- a) providing nucleic acid molecules that are (i) target nucleic acid molecules in said sample, or (ii) probes that hybridize to target nucleic acid molecules in said sample, or (iii) amplification products of (i) or (ii), or (iv) a genomic representation of (i);
- and
- b) detecting target nucleic acid molecules by contacting or comparing the nucleic acid molecules of (a) with a detection ensemble that has a minimum genomic derivation of greater than five and that comprises detection sequences that can detect target nucleic acid molecules.
A kit for obtaining genetic information from a biological sample, comprising:
- a) a plurality of ID probes and/or SNP probes;
- and
- b) a detection ensemble comprising detection sequences that are congruent with probes of (a), wherein said detection ensemble has a minimum genomic derivation of greater than five.
An ensemble of ID probes that can be amplified using fewer than four pairs of amplification sequences and that comprises more than three families of ID probes and more than ten different ID probes.
A method for obtaining genetic information from a biological sample potentially comprising target nucleic acid molecules, said method comprising the steps of:
- a) providing an ensemble of nucleic acid probes having a minimum genomic derivation of greater than five;
- b) contacting said ensemble of probes, simultaneously, with nucleic acid molecules of said sample;
- c) detecting hybridization between said probes and any target nucleic acid molecules of said sample;
- and
- d) identifying nucleic acid molecules detected in step (c).
A kit for obtaining genetic information from a biological sample, comprising
- a) a plurality of nucleic acid primers that are capable of priming the amplification of DNA sequences flanked by repetitive sequences in target genomic DNA in a biological sample to yield ID probes;
- and
- b) a detection ensemble comprising detection sequences that are congruent with ID probes potentially amplified using the primers of (a), wherein said detection ensemble has a minimum genomic derivation of greater than five.
Dependencies are inferred from the claim text.