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US 2003/0228583 A1Published application
Biomarkers of liver response
OwnerDavid E. Amacher, Lisa M. Fasulo +3inventors
InventorsDavid E. Amacher, Lisa M. Fasulo, Herath Mudiyanselage Athula Chandrasiri Herath, Gordon Duane Holt, Thomas R. Stiger
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Abstract
The present invention provides methods and compositions for screening, diagnosing and prognosing liver response, for monitoring the effectiveness of liver response treatment, and for drug development.
Claims18
A method for:
- (i) screening or diagnosing a liver response in a subject, (ii) determining the stage or severity of a liver response in a subject, (iii) identifying a subject at risk of developing liver response, or (iv) monitoring the effect of therapy administered to a subject having liver response, said method comprising detecting within a test biological sample from the subject a Liver Response-Associated Protein Isoforms (LRPIs) selected from the group consisting of:
- LRPI-1.1, LRPI-2.1, LRPI-3.1, LRPI-4.1, LRPI-6.1, LRPI-8.1, LRPI-9.1, LRPI-10.1, LRPI-11.1, LRPI-11.2, LRPI-11.3, LRPI-12.1, LRPI-13.1, LRPI-14.1, LRPI-15.1, LRPI-16.1, LRPI-17.1, LRPI-17.2, LRPI-1 8.1, LRPI-19.1, LRPI-20.1, LRPI-21.1, LRPI-22.1, LRPI-23.1, LRPI-24.1, LRPI-25.1, LRPI-26.1, LRPI-27.1, LRPI-28.1, LRPI-29.1, LRPI-30.1, LRPI-31.1, LRPI-32.1, LRPI-36.1, LRPI-37.1, LRPI-38.1, LRPI-53.1, LRPI-54.1, LRPI-56.1, LRPI-56.2, LRPI-60.1, LRPI-61.1, LRPI-63.1, LRPI-64.1, LRPI-66.1, LRPI-67.1, LRPI-69.1, LRPI-70.1, LRPI-71.1, LRPI-72.1, LRPI-73.1, LRPI-74.1, LRPI-75.1, LRPI-75.2, LRPI-76.1, LRPI-77.1, LRPI-78.1, LRPI-79.1, LRPI-79.2, LRPI-80.1, LRPI-81.1, LRPI-83.1, LRPI-84.1, LRPI-85.1, LRPI-87.1, LRPI-88.1, LRPI-89.1,LRPI-90.1,LRPI-91.1,LRPI-92.1,LRPI-107.1, LRPI-109.1,LRPI-110.1,LRPI-111.1, LRPI-112.1, LRPI-112.2, LRPI-113.1, LRPI-114.1, LRPI-115.1, LRPI-115.2, LRPI-119.1, LRPI-119.2, LRPI-119.3, LRPI-120.1, LRPI-121.1, LRPI-122.1, LRPI-123.1, LRPI-123.2, LRPI-124.1, LRPI-126.1, LRPI-127.1, LRPI-128.1, LRPI-129.1, LRPI-130.1, LRPI-131.1, LRPI-132.1, LRPI-132.2, LRPI-133.1, LRPI-134.1, LRPI-156.1, LRPI-157.1, LRPI-158.1, LRPI-159.1, LRPI-160.1, LRPI-161.1, LRPI-162.1, LRPI-163.1, LRPI-164.1, LRPI-165.1, LRPI-166.1, LRPI-167.1, LRPI-168.1, LRPI-169.1, LRPI-170.1, LRPI-172.1, LRPI-173.1, LRPI-174.1, LRPI-175.1, LRPI-175.2, LRPI-176.1, LRPI-177.1, LRPI-178.1, LRPI-179.1, LRPI-181.1, LRPI-182.1, LRPI-183.1, LRPI-184.1, LRPI-184.2, LRPI-217.1, LRPI-217.3, LRPI-218.1, LRPI-219.1, LRPI-220.1, LRPI-221.1, LRPI-222.1, LRPI-223.1, LRPI-224.1, LRPI-225.1, LRPI-226.1, LRPI-227.1, LRPI-228.1, LRPI-230.1, LRPI-231.1, LRPI-232.1, LRPI-232.2, LRPI-234.1, LRPI-235.1, LRPI-236.1, LRPI-237.1, LRPI-256.1, LRPI-257.1, LRPI-258.1, LRPI-259.1, LRPI-260.1, LRPI-261.1, LRPI-262.1, LRPI-263.1, LRPI-264.1, LRPI-265.1, LRPI-266.1, LRPI-270.1, LRPI-271.1, LRPI-294.1, LRPI-295.1, LRPI-296.1, LRPI-297.1, LRPI-298.1, LRPI-300.1, LRPI-300.2, LRPI-301.1, LRPI-303.1, LRPI-305.1, LRPI-306.1, LRPI-328.1, LRPI-329.1, LRPI-331.1, LRPI-332.1, LRPI-334.1, LRPI-335.1, LRPI-356.1, LRPI-358.1, LRPI-359.1, LRPI-360.1, LRPI-361.1, LRPI-363.1, LRPI-364.1, LRPI-365.1, LRPI-366.1, LRPI-367.1, LRPI-368.1, LRPI-369.1, LRPI-370.1, LRPI-371.1, LRPI-372.1, LRPI-373.1, LRPI-386.1, LRPI-387.1, LRPI-400.1, LRPI-400.2, LRPI-389.1, LRPI-390.1, LRPI-391.1, LRPI-392.1, LRPI-393.1, LRPI-394.1, LRPI-394.2, LRPI-395.1, LRPI-395.2, LRPI-397.1, LRPI-399.1, LRPI-401.1, LRPI-402.1, LRPI-403.1, LRPI-405.1, LRPI-405.2, LRPI-407.1, LRPI-409.1, LRPI-410.1, which has an activity or level indicative of a liver response.
A method for determining whether an agent induces a liver response comprising:
- a) exposing a target cell, tissue or biological organism to the agent;
- b) obtaining a test biological sample comprised of the target cell, tissue or biological organism;
- c) detecting and/or quantifying the level or activity of an LRPI in the sample;
- and
- d) comparing the level or activity of the LRPI in the test sample with the level or activity of said LRPI in a control sample, or with a previously determined reference range for said LRPI in targets free from liver response, or with the level or activity of at least one ERF in the test sample.
2 more elements
A kit comprising one or more antibodies capable of immunospecific binding to a Liver Response-Associated Protein Isoform (LRPI)s, other reagents and instructions for use.
A method for screening or diagnosing of liver response in a subject or for monitoring the effect of a drug or therapy administered to a subject, comprising:
- (a) contacting at least one oligonucleotide probe comprising 10 or more consecutive nucleotides complementary to a nucleotide sequence encoding an LRPI with RNA obtained from a biological sample from the subject or with cDNA copied from the RNA wherein said contacting occurs under conditions that permit hybridization of the probe to the nucleotide sequence if present;
- (b) detecting hybridization, if any, between the probe and the nucleotide sequence;
- and
- (c) comparing the hybridization, if any, detected in step (b) with the hybridization detected in a control sample, or with a previously determined reference range.
1 more element
Dependencies are inferred from the claim text.