Inhibitory immunoglobulin polypeptides to human PDGF beta receptor

An isolated nucleic acid having a sequence substantially identical to a nucleic acid coding for an immunoglobulin polypeptide or a binding fragment thereof, wherein binding of the polypeptide or fragment to a human βPDGF-R has one or more of the following effects:

• i) inhibition of PDGF BB or AB binding to the βPDGF-R;
• ii) inhibition of PDGF-induced βPDGF-R phosphorylation;
• iii) inhibition of PDGF-induced dimerization of βPDGF-R;
• iv) inhibition of PDGF-induced mitogenesis of cells displaying the human βPDGF-R;
• and
• v) inhibition of PDGF-induced chemotaxis and migration of cells displaying βPDGR-R.

A method of producing a substantially purified immunoglobulin polypeptide, or binding fragment thereof, which binds to a human type beta PDGF receptor (βPDGF-R), wherein the binding of the polypeptide or fragment to the βPDGF-R has one or more of the following effects:

• inhibition of PDGF BB or AB binding to the βPDGF-R;
• inhibition of PDGF-induced βPDGF-R phosphorylation;
• inhibition of PDGF-induced dimerization of the βPDGF-R;
• and inhibition of PDGF-induced mitogenesis of cells displaying human βPDGF-R;
• and inhibition of PDGF-induced chemotaxis and migration of cells displaying human β-PDGF;
• the method comprising:
• i) growing a cell line comprising a nucleic acid encoding the immunoglobulin polypeptide;
• and
• ii) harvesting the immunoglobulin polypeptide.