Method for scanning microscopy, scanning microscope, and apparatus for coding an illuminating light beam
AssigneeLeica Microsystems Heidelberg GmbH
InventorsRafael Storz, Volker Seyfried
Life of this patent
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Abstract
In a method for scanning microscopy an illuminating light beam that contains at least first light of a first wavelength and second light of a second wavelength, is coded. The coded illuminating light beam is directed onto a specimen and detection light proceeding from the specimen is decoded.
Claims21
A method for scanning microscopy comprising the steps of:
- generating an illuminating light beam that contains at least first light of a first wavelength and second light of a second wavelength, at least the first light comprising a coding;
- illuminating a specimen with the illuminating light beam;
- and
- decoding the detection light proceeding from the specimen.
A scanning microscope comprising at least one light source for generating an illuminating light beam that is directable onto a specimen and that contains at least first light of a first wavelength and second light of a second wavelength, a coding apparatus for coding at least the first light, and a decoding apparatus for decoding the detection light proceeding from the specimen.
A confocal scanning microscope having at least one light source for generating an illuminating light beam that is directable onto a specimen and that contains at least first light of a first wavelength and second light of a second wavelength, a coding apparatus for coding at least the first light, and a decoding apparatus for decoding the detection light proceeding from the specimen.
An apparatus for coding a illuminating light beam that contains at least first light of a first wavelength and second light of a second wavelength, comprising a beam splitter that splits the illuminating light beam into a first and a second sub-beam, a beam combiner that at least partially combines the first and the second sub-beam, are provided, and an acoustooptical component or an electrooptical component acting at least on the first sub-beam.
Use of an acoustooptical component for coding an illuminating light beam.
Dependencies are inferred from the claim text.