Methods for delivering nucleic acid molecules into cells and assessment thereof
OwnerGary de Jong, Edward Perkins +2inventors
InventorsGary de Jong, Edward Perkins, Adele Telenius, Neil MacDonald
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Abstract
Methods for delivering nucleic acid molecules into cells and methods for measuring nucleic acid delivery into cells and the expression of the nucleic acids are provided. The methods are designed for introduction of large nucleic acid molecules, including artificial chromosomes, into cells.
Claims84
A method for introducing a large nucleic acid molecule into a cell, comprising:obtaining a cell that is in a pre-selected phase;
- and
- contacting the cell with the large nucleic acid molecule, whereby the large nucleic acid molecule is delivered into the cell.
A method for introducing a chromosome or functional fragment thereof into a cell, comprising contacting the cell with a chromosome or fragment thereof that is in the same or similar phase as the cell, whereby the chromosome or functional fragment thereof is delivered into the cell.
A method for ex vivo gene therapy, comprising:
- obtaining a cell that is in a pre-selected phase;
- contacting the cell with a nucleic acid molecule, whereby the nucleic acid molecule is delivered into the cell;
- and introducing the cell into a subject.
A method for ex vivo gene therapy, comprising:
- contacting a cell with a chromosome or fragment thereof that is in the same or similar phase as the cell, whereby the chromosome or functional fragment thereof is delivered into the cell;
- and
- introducing the cell into a subject.
A kit for delivering large nucleic acids into cells, comprising:
- a delivery agent;
- a cell cycle arrest agent;
- and
- optionally instructions for delivering large nucleic acids into cells.
A method for selecting a host cell for receiving a large nucleic acid molecule, comprising:
- introducing a large nucleic acid molecule into cells at different phases;
- determining and comparing the efficiency of delivery and/or transfection of the nucleic acid into the cells at different phases;
- and
- selecting a cell that is in a phase that provides for increased efficiency of delivery and/or transfection relative to the efficiency of delivery and/or transfection at other phases.
Dependencies are inferred from the claim text.