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US 2003/0224476 A1Published application
Method of producing transglutaminase reactive compound
OwnerSzu-Yi Chouinventor
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Abstract
A method for producing transglutaminase-reactive compounds is provided. In one aspect, transglutaminase reactivity of a compound is enhanced. In another aspect, transglutaminase non-reactive compounds are modified to be reactive with transglutaminase.
Claims36
A method for enhancing transglutaminase reactivity, comprising:
- obtaining a compound;
- denaturing the compound in the presence of a denaturant;
- refolding the compound;
- and
- reacting the compound with a transglutaminase, wherein the transglutaminase reactivity for the compound is enhanced.
2 more elements
A method for enhancing transglutaminase reactivity, comprising:
- obtaining a compound;
- attaching at least one glutamine residue to the compound;
- denaturing the compound in the presence of a denaturant;
- refolding the compound;
- and
- reacting the compound with a transglutaminase, wherein the transglutaminase reactivity for the compound is enhanced.
3 more elements
A method for enhancing transglutaminase reactivity, comprising:
- obtaining a compound;
- attaching at least one glutamine residue to the compound;
- preparing the compound in a cross-linking solution;
- combining the compound cross-linking solution with a solution of a transglutaminase into a mixture;
- and
- incubating the mixture at a temperature for a period of time sufficient to effect and enhance transglutaminase reactivity to the compound.
3 more elements
A synthetic peptide composition reactive to transglutaminase, comprising:
- at least one glutamine residue.
A synthetic peptide composition reactive to transglutaminase, comprising:
- at least one glutamine residue at one terminus;
- and
- at least one lysine residue at the other terminus.
Dependencies are inferred from the claim text.