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US 2003/0224404 A1Published application
High throughput directed evolution of nucleic acids by rational mutagenesis
OwnerManuel Vega, Lila Drittantiinventors
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Abstract
Method and systems for the high throughput directed evolution of functional nucleic acid molecules, such as cis-acting elements, particularly those that act in complex biological settings, are provided.
Claims34
A method for the production of a molecule, having a predetermined property, comprising:
- (a) producing a population of sets of target functional nucleic acid molecules that each comprise a target modified functional sequence of nucleotides;
- (b) introducing each set of nucleic acid molecules into host cells and expressing a protein whose expression is modulated or regulated by the target functional sequence of nucleotides, wherein the host cells are present in an addressable collection;
- and
- (c) individually screening the sets of encoded proteins to identify the target functional nucleic acid molecules whose activity is altered, wherein each such target functional nucleic acid is designated a hit.
1 more element
A method for the production of a functional nucleic acid molecule having a predetermined property, comprising:
- (a) producing a population of sets of modified nucleic acid molecules that encode modified forms of a target functional nucleic acid, wherein each modified functional nucleic acid molecule is operably associated with a nucleic acid region encoding a reporter;
- (b) introducing each set of nucleic acid molecules into host cells under conditions that express a reporter when using a wild-type functional nucleic acid region, wherein the host cells are present in an addressable collection;
- (c) individually screening the sets of nucleic acid molecules encoding reporter proteins to identify one or more target modified functional nucleic acid regions that has activity that differs from the unmodified functional nucleic acid molecule, wherein each such molecule is designated a hit.
Dependencies are inferred from the claim text.