Compounds for hormonal therapy
OwnerVen Subbiahinventor
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Abstract
27-deoxyactein form is provided in high purity using a multi-step process. The plant of the Cimicifuga genus (e.g., the root portion) is contacted with an organic solvent to extract certain components, including triterpene glycosides, from that plant. The resulting extracted portion is subjected to a reduction step to convert some of the triterpene glycosides to 27-deoxyactein. The 27-deoxyactein is then isolated, preferably by chromatographic techniques. The present invention also provides a therapeutic composition comprising a therapeutically effective amount of the isolated 27-deoxyactein according to the above method.
Claims44
A process for providing 27-deoxyactein from at least a portion of a plant of the Cimicifuga genus, the process comprising:
- (a) contacting at least a portion of the plant with an organic solvent to provide a mixture of the plant portion and the organic solvent;
- (b) subjecting the mixture to conditions sufficient to separate a solvent insoluble plant portion from an extracted plant portion within the solvent;
- (c) subjecting the extracted plant portion to conditions sufficient to reduce the extracted plant portion;
- and
- (d) separating 27-deoxyactein from the reduced extracted plant portion.
A process of isolating a 27-deoxyactein from at least a portion of a black cohosh plant, the process comprising:
- (a) contacting at least a portion of the black cohosh plant with an organic solvent to provide a slurry of the plant portion and the organic solvent;
- (b) subjecting the slurry to conditions sufficient to separate a solvent insoluble plant portion from an extracted plant portion within the solvent;
- (c) providing the extracted plant portion in solid form;
- (d) contacting the extracted plant portion with a reducing agent in a solvent under conditions sufficient to provide reduced triterpene glycoside components of the extracted plant portion;
- (e) separating the reduced extracted triterpene glycoside components from the reducing solvent using a solvent different from the reducing solvent to isolate those triterpene glycoside components;
- (f) separating 27-deoxyactein from the triterpene glycoside components using chromatographic techniques;
- and
- (g) isolating 27-deoxyactein in substantially pure form.
27-deoxyactein in substantially pure form.
A method of treating hormonal imbalance conditions in a patient in need of treatment, the method comprising administering to the patient a therapeutically effective amount of substantially pure 27-deoxyactein isolated from a portion of a plant of the Cimicifuga genus.
A pharmaceutical composition useful in the treatment of hormonal imbalance conditions, the composition comprising a therapeutically effective amount of substantially pure 27-deoxyactein isolated from a portion of a plant of the Cimicifuga genus.
A process of providing 27-deoxyactein from at least a portion of a plant of the Cimicifuga genus, the process comprising:
- (a) contacting at least a portion of the plant with an organic solvent to provide extracted plant portion within the solvent;
- (b) subjecting the extracted plant portion to conditions sufficient to reduce the extracted plant portion;
- and
- (c) separating 27-deoxyactein from the reduced extracted plant portion.
Dependencies are inferred from the claim text.