Methods for entrapment of bioactive agent in a liposome or lipid complex
AssigneeTransave, Inc.
InventorsLawrence T. Boni, Brian Miller, Fangjun Wu
Life of this patent
Estimating the term…
Abstract
A method of preparing a liposomal bioactive agent comprising infusing an lipid-ethanol mixture with an aqueous or ethanolic solution of the bioactive agent at a temperature below the phase transition of at least one of the lipid components of the lipid and compositions produced by the method of the invention.
Claims53
A method of preparing a liposomal bioactive agent comprising infusing an lipid-ethanol mixture with an aqueous or ethanolic solution of the bioactive agent at a temperature below the phase transition of at least one of the lipid components of the lipid.
The method of claim 17, wherein the temperature is below approximately 40 degrees Celsius.
The method of claim 17, wherein the temperature is below approximately 30 degrees Celsius.
A method of entrapment of a bioactive agent in a liposome or lipid complex comprising the steps of:
- a) preparing an aqueous or ethanolic solution containing the bioactive agent;
- b) preparing an lipid-ethanol solution;
- and,
- c) infusing the lipid-ethanol solution into the aqueous or ethanolic solution containing the bioactive agent to produce a product, wherein the step of infusing is performed at a temperature below the phase transition of at least one of the lipid components of the lipid-ethanol solution.
A method of entrapment of a bioactive agent in a liposome or lipid complex comprising the steps of:
- a) preparing a aqueous or ethanolic solution containing the bioactive agent;
- b) preparing small unilamellar vesicles;
- c) mixing the aqueous or ethanolic solution containing the bioactive agent with the small unilamellar vesicles to make a resultant solution,
- d) infusing ethanol into the resultant solution to produce a product, wherein the step of infusing is performed at a temperature below the phase transition of at least one of the lipid components of the lipid-ethanol solution.
Dependencies are inferred from the claim text.