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US 2003/0224020 A1Published application
Pharmaceutical preparation of botulinum neurotoxin, methods of synthesis and methods of clinical use
OwnerAlexander F. Zabudkin, Juri M. Krasnopolsky +2inventors
InventorsAlexander F. Zabudkin, Juri M. Krasnopolsky, Aleksandr M. Itkin, Dmitry M. Itkin
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Abstract
A pharmaceutical preparation of botulinum neurotoxins free of human blood products (such as human albumin), the preparation comprising a botulinum neurotoxin incorporated in phosphatidylcholine liposomes.
Claims25
A pharmaceutical preparation comprising:
- (a) botulinum neurotoxin;
- (b) lactose;
- and
- (c) phosphatidylcholine effectively made into a liposome which incorporates said botulinum neurotoxin.
1 more element
A method of making a pharmaceutical preparation, the method comprising:
- (a) providing a solution of phosphatidylcholine in ethanol;
- (b) subjecting said solution of phosphatidylcholine in ethanol to evaporation until a lipid film is formed;
- (c) passing an inert gas through the lipid film;
- (d) re-suspending said lipid film in a sterile chloride solution with phosphate buffer contain botulinum neurotoxin creating an emulsion;
- (e) homogenizing said emulsion;
- and
- (f) sequentially filtering the homogenized emulsion.
4 more elements
A pharmaceutical preparation consisting essentially of:
- (a) botulinum neurotoxin;
- (b) lactose;
- and
- (c) phosphatidylcholine effectively made into a liposome which incorporates said botulinum neurotoxin.
1 more element
Dependencies are inferred from the claim text.