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US 2003/0223983 A1Published application
Formulation comprising bioactive agents and method of using same
AssigneesUniversity of Erlangen-Nurnberg, Wyeth
InventorsSusan J. Sofia, Daniel Schwartz, Wolfgang Friess
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Abstract
Disclosed are methods of making bioactive BMP-2 microparticles and methods of using the microparticles.
Claims35
A purified microparticle comprising a precipitated bioactive bone morphogenetic protein 2 (BMP-2).
A pharmaceutical composition comprising a precipitated bioactive bone morphogenetic protein 2 (BMP-2) microparticle and a pharmaceutically acceptable carrier.
A method of increasing bone morphogenetic protein-2 (BMP-2) levels in a subject, the method comprising
- providing an aqueous BMP-2 suspension comprising a resolubilized BMP-2 polypeptide;
- and
- introducing said aqueous suspension into said subject in an amount sufficient to increase BMP-2 levels in said subject.
A method of making a population of bioactive BMP-2 microparticles, the method comprising
- providing a first solution comprising a BMP-2 polypeptide at a concentration of about 0.75 mg/ml to 2.5 mg/ml and a pH of about 4.2 to about 4.8;
- mixing said first solution with a second solution with a known pH and ionic concentration that results in precipitation of BMP-2 polypeptide upon contact with said second solution, thereby forming a mixed first solution and second solution;
- and
- allowing BMP-2 polypeptides to precipitate, thereby forming a population of BMP-2 microparticles.
1 more element
Dependencies are inferred from the claim text.