Control sequences of the human corin gene
AssigneeSchering Aktiengesellchaft
InventorsJunliang Pan, Qingyu Wu
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Abstract
This invention provides a novel expression control region isolated from mammalian corin genes. This control region preferentially activates transcription in cardiac cells. Methods and compositions are provided to employ this control region for identification of agents capable of modulating corin expression and for treatment of cardiac disease.
Claims28
An isolated polynucleotide comprising a mammalian corin expression control region, wherein the control region modulates the transcription of a heterologous polynucleotide to which it is operably linked.
A method of identifying an agent which modulates the expression of a human corin gene in a cell, wherein the method comprises:
- (a) producing a recombinant vector in which an isolated polynucleotide comprising a mammalian corin expression control region is operably linked to a reporter gene;
- (b) transfecting the cell with the recombinant vector;
- (c) treating the cell with the agent;
- (d) measuring the level of expression of the reporter sequence in the treated cell;
- and
- (e) comparing the level of expression of the reporter sequence in the presence of the agent to the level of expression in an transfected control cell which has not been treated
A method for modulating the expression of a gene in a human subject, the method comprising:
- (a) producing a recombinant vector in which an isolated polynucleotide comprising a mammalian corin expression control region is operably linked to a heterologous polynucleotide;
- (b) administering the vector in a therapeutically effective amount to the subject.
A method for treating congestive heart failure, hypertension or myocardial infarction in a human subject, the method comprising administering a therapeutically effective amount of an isolated polynucleotide comprising a mammalian corin expression control region, operably linked to a gene selected from the group consisting of corin, ANP, B-type natriuretic peptide, phospholamban, ACE, or negative dominant forms of these genes, to the subject.
Dependencies are inferred from the claim text.