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OwnerAllen James Ebens JR., Kevin Patrick Keegan +1inventors
InventorsAllen James Ebens JR., Kevin Patrick Keegan, Thomas J Stout
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Nucleic acids isolated from Drosophila melanogaster that are lethal when knocked out in Drosophila, and proteins encoded thereby, are described. The nucleic acids and proteins can be used to genetically modify metazoan invertebrate organisms, such as insects and worms, or cultured cells, resulting in expression or mis-expression of the encoded proteins. The genetically modified organisms or cells can be used in screening assays to identify candidate compounds which are potential pesticidal agents or therapeutics that interact with subject proteins. They can also be used in methods for studying activity of subject proteins, and identifying other genes that modulate the function of, or interact with, the subject genes.
An isolated nucleic acid molecule of less than about 15 kb in size comprising a nucleic acid sequence that encodes an invertebrate receptor polypeptide and that shares at least about 75% nucleotide sequence identity with the sequence set forth in SEQ ID NO:1, or the complement thereof.
An isolated nucleic acid molecule of less than about 15 kb in size comprising a nucleic acid sequence that encodes an invertebrate receptor polypeptide and that shares at least about 75% nucleotide sequence identity with the sequence set forth in SEQ ID NO:3, or the complement thereof.
An isolated nucleic acid molecule of less than about 15 kb in size comprising a nucleic acid sequence that encodes an invertebrate receptor polypeptide and that shares at least about 75% nucleotide sequence identity with the sequence set forth in SEQ ID NO:5, or the complement thereof.
An isolated nucleic acid molecule comprising a nucleic acid sequence that encodes a polypeptide comprising at least 36 amino acids that share 100% sequence identity with 36 contiguous amino acids of SEQ ID NO:2.
An isolated nucleic acid molecule comprising a nucleic acid sequence that encodes a polypeptide comprising at least 27 amino acids that share 100% sequence identity with 27 contiguous amino acids of SEQ ID NO:4.
An isolated nucleic acid molecule comprising a nucleic acid sequence that encodes a polypeptide comprising at least 46 amino acids that share 100% sequence identity with 46 contiguous amino acids of SEQ ID NO:6.
A purified protein comprising an amino acid sequence having at least about 80% sequence identity with any one of the sequences set forth in SEQ ID NOS:2, 4, or 6.
A method for detecting a candidate compound that interacts with a helicase protein or fragment thereof, said method comprising contacting said helicase protein or fragment with one or more candidate molecules, and detecting any interaction between said candidate compound and said helicase protein or fragment;
A method for detecting a candidate compound that interacts with a phosphatidylinositol transfer protein (PITP) or fragment thereof, said method comprising contacting said PITP or fragment with one or more candidate molecules, and detecting any interaction between said candidate compound and said PITP or fragment;
A method for detecting a candidate compound that interacts with a sphingosine phosphate lyase (SPL) or fragment thereof, said method comprising contacting said SPL or fragment with one or more candidate molecules, and detecting any interaction between said candidate compound and said SPL or fragment;
A first animal that is an insect or a worm that has been genetically modified to express or mis-express a protein, or the progeny of said animal that has inherited said protein expression or mis-expression, wherein said protein comprises an amino acid sequence that shares at least about 80% identity with a sequence as set forth in any of SEQ ID NOS:2, 4, or 6.
Dependencies are inferred from the claim text.